Evidence map›Paper›PMID 32342199›Full record

ArticleEuropean archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery2020

ATF5 involved in radioresistance in nasopharyngeal carcinoma by promoting epithelial-to-mesenchymal phenotype transition.

Yu Shuai, Erxi Fan, Qiuyue Zhong, Guangyong Feng, Qiying Chen, Xiaoxia Gou, Guihai Zhang

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yu ShuaiDepartment of Head and Neck Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, People's Republic of China.
Erxi FanDepartment of Head and Neck Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, People's Republic of China.
Qiuyue ZhongDepartment of Head and Neck Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, People's Republic of China.
Guangyong FengDepartment of Head and Neck Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, People's Republic of China.
Qiying ChenDepartment of Head and Neck Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, People's Republic of China.
Xiaoxia GouDepartment of Head and Neck Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, People's Republic of China.
Guihai ZhangDepartment of Head and Neck Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, People's Republic of China. zghzhuhai@163.com.
Zunyi Medical University · CNZhuhai People's Hospital · CN

Funding

National Natural Science Foundation of China 81260370
6 · The paper itself

Abstract

purposeThis study aimed to investigate the effects of activating transcription factor-5 (ATF5) on nasopharyngeal carcinoma (NPC) cell radioresistance.

methodsHONE-1 nasopharyngeal carcinoma cells were irradiated by conventional fractionation to generate HONE-1R radiotherapy-resistant cells. Short hairpin RNA (shRNA) expression plasmids targeting the ATF5 gene were constructed and transfected into the HONE-1R cell line. The proliferation assay, colony formation analysis, Transwell Boyden chamber assay and other experimental methods were performed to verify changes in the radiosensitivity and other biological of NPC cells.

resultsX-ray irradiation significantly promoted the upregulation of ATF5 protein levels in HONE-1 cells, and the protein expression of ATF5 increased with the dose of X-ray irradiation (p < 0.05). The colony formation rate, cell survival rate and migration ability of HONE-1R cells were significantly higher than those of HONE-1 cells (p < 0.05). Simultaneously, X-ray could also promote the morphology of epithelial-mesenchymal transition (EMT) in HONE-1 cells, inducing a lower expression level of E-cadherin and a higher expression level of N-cadherin in a dose-dependent manner (p < 0.05). Inhibiting ATF5 significantly reduced the colony formation rate, cell survival rate, migration and invasiveness of HONE-1R cells (p < 0.05). Moreover, sensitizing HONE-1R cells to X-ray irradiation significantly upregulated the expression of E-cadherin and downregulated the expression of N-cadherin in these cells (p < 0.05).

conclusionsATF5 may be a potential therapeutic target to improve radiosensitivity in NPC.

Indexed as

CarcinomaNasopharyngeal NeoplasmsActivating Transcription FactorsCell Line, TumorCell MovementEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHumansNasopharyngeal CarcinomaPhenotypeActivating Transcription FactorsATF5 protein, humanActivating transcription factor-5Nasopharyngeal carcinomaRadiotherapy resistance

Identifiers

PMID32342199
OpenAlexW3019697274

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.