ArticleNature methods2020
A comparison of microfluidic methods for high-throughput cell deformability measurements.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
123 citing papers in PubMed, 269 citations in OpenAlex.
- Simulation-Based Microfluidic Deformation Mapping for Region-Dependent Apparent Young's Modulus Estimation of Single Cells.Biosensors · 2026Article
- Real-Time Biophysical Phenotyping and Sorting of Transiting Cells Along a Constriction Microchannel.Micromachines · 2026Article
- Mechanistic insights into cellular deformation enable enhanced extensional-flow cytometry for label-free classification and sorting.Microsystems & nanoengineering · 2026Article
- A parametric study of mechanoporation through microfluidic design to modulate shear, compressive, and adhesion forces and loading rates.Lab on a chip · 2026Article
- Automation and improvement of WBC mechanical profiling in deformability cytometry.Biophysical journal · 2026Article
- High-throughput mechanomic screening reveals novel regulators of single-cell mechanics.Biophysical journal · 2026Article
- Transformative biomechanics and mechanobiology breakthroughs shaping the future of health and medicine.Innovation (Cambridge (Mass.)) · 2026Review
- Rapid-manufacturing and cost-effective single-layer microfluidic device for high-throughput three-dimensional hydrodynamic focusing.Microsystems & nanoengineering · 2026Article
- Mechanomedicine.Nature reviews bioengineering · 2026Article
- Label-Free Leukocyte Biophysical Profiling Using Impedance-Deformability Cytometry for Rapid Cardiovascular Risk Stratification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A Mechanophenotyping chip for high-throughput detection of metastatic bacteria-infected circulating tumor cells.Nature communications · 2026Article
- Impact of staining on cell acoustic properties.Biophysical journal · 2026Article
- Mechanobiological regulation of T cellsLab on a chip · 2026Article
- DEP-track: a motion-aware framework for large-scale cell tracking and crossover frequency estimation in dielectrophoresis.Frontiers in bioinformatics · 2026Article
- Brillouin Microscopy of Breast tumor Spheroids On-a-Chip: Mechanical and Transcriptional Responses to Microfluidic-Induced Rapid Deformations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Multiscale measurement of brain tissue and cell biomechanics using a mouse model.Biophysics reports · 2025Article
- Effects of intravenous lipid emulsions on Jurkat cells assessed using label-free deformability cytometry.Scientific reports · 2025Article
- Lab on an end: Micromanipulation using the acoustohydrodynamic pillar array as an end effector.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses.FEBS letters · 2025Review
- Label-Free Cancer Detection Methods Based on Biophysical Cell Phenotypes.Bioengineering (Basel, Switzerland) · 2025Review
63 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
The mechanical phenotype of a cell is an inherent biophysical marker of its state and function, with many applications in basic and applied biological research. Microfluidics-based methods have enabled single-cell mechanophenotyping at throughputs comparable to those of flow cytometry. Here, we present a standardized cross-laboratory study comparing three microfluidics-based approaches for measuring cell mechanical phenotype: constriction-based deformability cytometry (cDC), shear flow deformability cytometry (sDC) and extensional flow deformability cytometry (xDC). All three methods detect cell deformability changes induced by exposure to altered osmolarity. However, a dose-dependent deformability increase upon latrunculin B-induced actin disassembly was detected only with cDC and sDC, which suggests that when exposing cells to the higher strain rate imposed by xDC, cellular components other than the actin cytoskeleton dominate the response. The direct comparison presented here furthers our understanding of the applicability of the different deformability cytometry methods and provides context for the interpretation of deformability measurements performed using different platforms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.