Evidence map›Paper›PMID 32341128›Full record

ArticleThe Journal of biological chemistry2020

A fluorescent timer reporter enables sorting of insulin secretory granules by age.

Belinda Yau, Lori Hays, Cassandra Liang, D Ross Laybutt, Helen E Thomas, Jenny E Gunton, Lindy Williams, Wayne J Hawthorne, Peter Thorn, Christopher J Rhodes and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Review
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  3. Article
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  5. Article
  6. Roles of molecular motors in insulin-secreting beta cells.Current opinion in cell biology · 2025
    Review
  7. Article
  8. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Belinda YauCharles Perkins Centre, University of Sydney, Sydney, New South Wales, Australia; School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, New South Wales, Australia.
Lori HaysSTEM-Department of Biology, Edmonds Community College, Lynnwood, Washington, USA.
Cassandra LiangGarvan Institute of Medical Research, Sydney, New South Wales, Australia.
D Ross LaybuttGarvan Institute of Medical Research, Sydney, New South Wales, Australia; St. Vincent's Clinical School, University of New South Wales Sydney, Sydney, New South Wales, Australia.
Helen E ThomasSt. Vincent's Institute, Fitzroy, Victoria, Australia; Department of Medicine, St. Vincent's Hospital, University of Melbourne, Fitzroy, Victoria, Australia.
Jenny E GuntonFaculty of Medicine and Health, the University of Sydney, Sydney, New South Wales, Australia; The Westmead Institute for Medical Research, University of Sydney, Westmead, New South Wales, Australia.
Lindy WilliamsFaculty of Medicine and Health, the University of Sydney, Sydney, New South Wales, Australia; National Pancreas and Islet Transplant Unit (NPITU), Westmead Hospital, Sydney, New South Wales, Australia.
Wayne J HawthorneFaculty of Medicine and Health, the University of Sydney, Sydney, New South Wales, Australia; National Pancreas and Islet Transplant Unit (NPITU), Westmead Hospital, Sydney, New South Wales, Australia.
Peter ThornCharles Perkins Centre, University of Sydney, Sydney, New South Wales, Australia; Discipline of Physiology, School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, University of Sydney, Camperdown, New South Wales, Australia.
Christopher J RhodesResearch and Early Development, Cardiovascular, Renal and Metabolic Diseases, BioPharmaceuticals R&D, AstraZeneca Ltd, Gaithersburg, Maryland, USA; Pacific Northwest Research Institute, Seattle, Washington, USA.
Melkam A KebedeCharles Perkins Centre, University of Sydney, Sydney, New South Wales, Australia; School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, New South Wales, Australia. Electronic address: melkam.kebede@sydney.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Within the pancreatic β-cells, insulin secretory granules (SGs) exist in functionally distinct pools, displaying variations in motility as well as docking and fusion capability. Current therapies that increase insulin secretion do not consider the existence of these distinct SG pools. Accordingly, these approaches are effective only for a short period, with a worsening of glycemia associated with continued decline in β-cell function. Insulin granule age is underappreciated as a determinant for why an insulin granule is selected for secretion and may explain why newly synthesized insulin is preferentially secreted from β-cells. Here, using a novel fluorescent timer protein, we aimed to investigate the preferential secretion model of insulin secretion and identify how granule aging is affected by variation in the β-cell environment, such as hyperglycemia. We demonstrate the use of a fluorescent timer construct, syncollin-dsRedE5TIMER, which changes its fluorescence from green to red over 18 h, in both microscopy and fluorescence-assisted organelle-sorting techniques. We confirm that the SG-targeting construct localizes to insulin granules in β-cells and does not interfere with normal insulin SG behavior. We visualize insulin SG aging behavior in MIN6 and INS1 β-cell lines and in primary C57BL/6J mouse and nondiabetic human islet cells. Finally, we separated young and old insulin SGs, revealing that preferential secretion of younger granules occurs in glucose-stimulated insulin secretion. We also show that SG population age is modulated by the β-cell environment

Indexed as

AnimalsCell LineExocytosisFluorescent DyesGlucoseHumansInsulinInsulin-Secreting CellsInsulin SecretionIslets of LangerhansMaleMembrane ProteinsMiceMice, Inbred C57BLMicroscopy, FluorescenceSecretory VesiclesFluorescent DyesGlucoseInsulinMembrane ProteinsSycn protein, ratdsRed-E5exocytosisfluorescent timergranule aginginsulininsulin secretioninsulin secretory granule (SG)type 2 diabetesvesiclesβ-cell

Identifiers

PMID32341128
PMCPMC7335792

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.