Evidence map›Paper›PMID 32341124›Full record

ArticleThe Journal of biological chemistry2020

Quantification of native mRNA dynamics in living neurons using fluorescence correlation spectroscopy and reduction-triggered fluorescent probes.

Hirotaka Fujita, Ryota Oikawa, Mayu Hayakawa, Fumiaki Tomoike, Yasuaki Kimura, Hiroyuki Okuno, Yoshiki Hatashita, Carolina Fiallos Oliveros, Haruhiko Bito, Toshio Ohshima and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Hirotaka FujitaDepartment of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Ryota OikawaDepartment of Chemistry, Graduate School of Science, Nagoya University, Aichi, Japan.
Mayu HayakawaDepartment of Chemistry, Graduate School of Science, Nagoya University, Aichi, Japan.
Fumiaki TomoikeDepartment of Chemistry, Graduate School of Science, Nagoya University, Aichi, Japan.
Yasuaki KimuraDepartment of Chemistry, Graduate School of Science, Nagoya University, Aichi, Japan.
Hiroyuki OkunoGraduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.ORCID 0000-0001-6237-6503
Yoshiki HatashitaDepartment of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Carolina Fiallos OliverosDepartment of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Haruhiko BitoDepartment of Neurochemistry, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.ORCID 0000-0001-6315-9594
Toshio OhshimaDepartment of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Satoshi TsunedaDepartment of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Hiroshi AbeDepartment of Chemistry, Graduate School of Science, Nagoya University, Aichi, Japan.
Takafumi InoueDepartment of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan inoue.t@waseda.jp.ORCID 0000-0002-2728-0060
Waseda University · JPNagoya University · JPKagoshima University · JPThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA localization in subcellular compartments is essential for spatial and temporal regulation of protein expression in neurons. Several techniques have been developed to visualize mRNAs inside cells, but the study of the behavior of endogenous and nonengineered mRNAs in living neurons has just started. In this study, we combined reduction-triggered fluorescent (RETF) probes and fluorescence correlation spectroscopy (FCS) to investigate the diffusion properties of activity-regulated cytoskeleton-associated protein (

Indexed as

AnimalsCells, CulturedCytoskeletal ProteinsFluorescent DyesInositol 1,4,5-Trisphosphate ReceptorsNerve Tissue ProteinsNeuronsOptical ImagingRatsRats, WistarRNA, MessengerSpectrometry, Fluorescenceactivity regulated cytoskeletal-associated proteinCytoskeletal ProteinsFluorescent DyesInositol 1,4,5-Trisphosphate ReceptorsItpr1 protein, ratNerve Tissue ProteinsRNA, Messengercellular dynamicsfluorescence correlation spectroscopy (FCS)gene regulationhippocampuslive-cell imagingmRNAneuronreduction-triggered fluorescence (RETF)RNA transporttranscription

Identifiers

PMID32341124
PMCPMC7278347
OpenAlexW3017551837

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.