Evidence map›Paper›PMID 32340348›Full record

ArticleCells2020

Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4

Flavia Franco da Cunha, Vinicius Andrade-Oliveira, Danilo Candido de Almeida, Tamiris Borges da Silva, Cristiane Naffah de Souza Breda, Mario Costa Cruz, Eliana L Faquim-Mauro, Marcos Antonio Cenedeze, Meire Ioshie Hiyane, Alvaro Pacheco-Silva and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Flavia Franco da CunhaDepartamento de Nefrologia, UNIFESP, Rua Pedro de Toledo 669, São Paulo 04039-032, Brazil.ORCID 0000-0001-8209-7421
Vinicius Andrade-OliveiraDepartamento de Imunologia, USP, Avenida Prof. Lineu Prestes 1730, ICB IV, São Paulo 05508-000, Brazil.
Danilo Candido de AlmeidaDepartamento de Nefrologia, UNIFESP, Rua Pedro de Toledo 669, São Paulo 04039-032, Brazil.
Tamiris Borges da SilvaDepartamento de Nefrologia, UNIFESP, Rua Pedro de Toledo 669, São Paulo 04039-032, Brazil.
Cristiane Naffah de Souza BredaDepartamento de Imunologia, USP, Avenida Prof. Lineu Prestes 1730, ICB IV, São Paulo 05508-000, Brazil.
Mario Costa CruzDepartamento de Imunologia, USP, Avenida Prof. Lineu Prestes 1730, ICB IV, São Paulo 05508-000, Brazil.
Eliana L Faquim-MauroLaboratório de Imunopatologia, Instituto Butantan, Av. Vital Brasil 1500, São Paulo 05503-900, Brazil.
Marcos Antonio CenedezeDepartamento de Nefrologia, UNIFESP, Rua Pedro de Toledo 669, São Paulo 04039-032, Brazil.
Meire Ioshie HiyaneDepartamento de Imunologia, USP, Avenida Prof. Lineu Prestes 1730, ICB IV, São Paulo 05508-000, Brazil.
Alvaro Pacheco-SilvaDepartamento de Nefrologia, UNIFESP, Rua Pedro de Toledo 669, São Paulo 04039-032, Brazil.
Regiane Aparecida CavinatoDepartamento de Nefrologia, UNIFESP, Rua Pedro de Toledo 669, São Paulo 04039-032, Brazil.
Ana Claudia TorrecilhasDepartamento de Ciências Farmacêuticas, UNIFESP, Rua São Nicolau 210, Diadema 09913-030, São Paulo, Brazil.ORCID 0000-0001-5724-2199
Niels Olsen Saraiva CâmaraDepartamento de Nefrologia, UNIFESP, Rua Pedro de Toledo 669, São Paulo 04039-032, Brazil.ORCID 0000-0001-5436-1248
Universidade Federal de São Paulo · BRHospital Israelita Albert Einstein · BRInstituto Butantan · BRInstituto de Ciências Farmacêuticas · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stromal cells (MSCs) can generate immunological tolerance due to their regulatory activity in many immune cells. Extracellular vesicles (EVs) release is a pivotal mechanism by which MSCs exert their actions. In this study, we evaluate whether mesenchymal stromal cell extracellular vesicles (MSC-EVs) can modulate T cell response. MSCs were expanded and EVs were obtained by differential ultracentrifugation of the supernatant. The incorporation of MSC-EVs by T cells was detected by confocal microscopy. Expression of surface markers was detected by flow cytometry or CytoFLEX and cytokines were detected by RT-PCR, FACS and confocal microscopy and a miRNA PCR array was performed. We demonstrated that MSC-EVs were incorporated by lymphocytes in vitro and decreased T cell proliferation and Th1 differentiation. Interestingly, in Th1 polarization, MSC-EVs increased Foxp3 expression and generated a subpopulation of IFN-γ

Indexed as

AnimalsCD4-Positive T-LymphocytesCell DifferentiationCell ProliferationExtracellular VesiclesForkhead Transcription FactorsGlycolysisMembrane Potential, MitochondrialMesenchymal Stem CellsMice, Inbred C57BLMicroRNAsSignal TransductionT-Lymphocytes, RegulatoryForkhead Transcription FactorsFoxp3 protein, mouseMicroRNAsextracellular vesiclesmesenchymal stromal cellsmetabolismmiRNATh1 polarization

Identifiers

PMID32340348
PMCPMC7226573
OpenAlexW3020795010

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.