Evidence map›Paper›PMID 32338463›Full record

ArticleAdvanced healthcare materials2020

Smart Material Hydrogel Transfer Devices Fabricated with Stimuli-Responsive Silk-Elastin-Like Proteins.

Rachael N Parker, Dana M Cairns, Wenyao A Wu, Kathryn Jordan, Chengchen Guo, Wenwen Huang, Zaira Martin-Moldes, David L Kaplan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds.Advanced materials (Deerfield Beach, Fla.) · 2024
    Review
  4. Review
  5. Contribution of the ELRs to the development of advancedFrontiers in bioengineering and biotechnology · 2024
    Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Review
  15. Patient-Derived Organoids as a Model for Cancer Drug Discovery.International journal of molecular sciences · 2021
    Review
  16. Article
  17. Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Rachael N ParkerDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA.
Dana M CairnsDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA.
Wenyao A WuDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA.
Kathryn JordanDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA.
Chengchen GuoDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA.
Wenwen HuangDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA.
Zaira Martin-MoldesDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA.
David L KaplanDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155, USA.ORCID 0000-0002-9245-7774
Tufts University · US

Funding

Tissue Engineering Resource Center: TTDP41EB027062 · NIBIB · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Gordana Vunjak-Novakovic · 2019 to 2026
$12.6M
Models to Predict Protein Biomaterial PerformanceU01EB014976 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI BUEHLER, MARKUS J., KAPLAN, DAVID L. · 2012 to 2020
$5.2M
CCR NIH HHS HHSN261200800001CNCI NIH HHS HHSN261200800001ENIBIB NIH HHS P41 EB027062NIBIB NIH HHS U01 EB014976
6 · The paper itself

Abstract

Three-dimensional organoid tissue culture models are a promising approach for the study of biological processes including diseases. Advances in these tissue culture technologies improve in vitro analysis compared to standard 2D cellular approaches and are more representative of the physiological environment. However, a major challenge associated with organoid systems stems from the laborious processing involved in the analysis of large numbers of organoids. Here the design, characterization, and application of silk-elastin-like protein-based smart carrier arrays for processing organoids is presented. Fabrication of hydrogel-based carrier systems at room temperature result in organized arrays of organoids that maintain tissue culture plate orientation and could be processed simultaneously for histology. The system works by transfer of the organoids to the hydrogel arrays after which the material is subjected to 65 °C to induce hydrogel contraction to secure the organoids, resulting in multisample constructs and allowing for placement on a microscope slide. Histological processing and immunostaining of these arrayed cerebral organoids analyzed within the contracted silk-elastin-like proteins (SELP) show retention of native organoid features compared to controls without the hydrogel carrier system, thus avoiding any artifacts. These SELP carriers present a useful approach for improving efficiency of scaled organoid screening and processing.

Indexed as

Biological PhenomenaSmart MaterialsElastinHydrogelsOrganoidsSilkElastinHydrogelsSilkSmart Materialshistologyhydrogelsorganoidssilk-elastin-like protein

Identifiers

PMID32338463
PMCPMC7466789
OpenAlexW3019677123

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.