ArticleMarine biotechnology (New York, N.Y.)2020
siRNA-Mediated MrIAG Silencing Induces Sex Reversal in Macrobrachium rosenbergii.
Article in Marine biotechnology (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Effects of RNA Interference-Mediated Silencing of the Insulin-Like Androgenic Gland Hormone Gene on Growth and Gonad Development in the Swimming Crab (Animals : an open access journal from MDPI · 2026Article
- Establishment of a CRISPR/Cas9-Based Genome Editing System inAnimals : an open access journal from MDPI · 2025Article
- Review
- Comprehensive Multi-Omics Analysis of Muscle Tissue Alterations in MaleInternational journal of molecular sciences · 2025Article
- Sex reversal induced by 17β-estradiol may be achieved by regulating the neuroendocrine system of the Pacific white shrimp Penaeus vannamei.BMC genomics · 2025Article
- Involvement of the ovarian-specific Mro-IR in oogonia differentiation and oocyte development in freshwater giant prawnFrontiers in endocrinology · 2025Article
- Molecular Approaches Detect Early Signals of Programmed Cell Death inCurrent issues in molecular biology · 2024Article
- Phylogenetic and transcriptomic characterization of insulin and growth factor receptor tyrosine kinases in crustaceans.Frontiers in endocrinology · 2024Article
- Article
- Analysis of mRNA and MicroRNA Expression Profiles of Nervous Tissues and Reproductive Tissues in Male Procambarus clarkii After Silencing IAG.Marine biotechnology (New York, N.Y.) · 2023Article
- Identification of Crustacean Female Sex Hormone Receptor Involved in Sexual Differentiation of a Hermaphroditic Shrimp.Biomolecules · 2023Article
- Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud CrabInternational journal of molecular sciences · 2023Article
- Identification of a Putative CFSH Receptor Inhibiting IAG Expression in Crabs.International journal of molecular sciences · 2023Article
- The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn.iScience · 2023Article
- Alterations of the Gut Microbiota and Metabolomics Associated with the Different Growth Performances ofAnimals : an open access journal from MDPI · 2023Article
- Article
- Identification and Expression Analysis ofInternational journal of molecular sciences · 2022Article
- Full Functional Sex Reversal Achieved Through Silencing ofFrontiers in endocrinology · 2021Article
- The "IAG-Switch"-A Key Controlling Element in Decapod Crustacean Sex Differentiation.Frontiers in endocrinology · 2020Review
- Molecular Characterization of the Insulin-Like Androgenic Gland Hormone in the Swimming Crab,Frontiers in endocrinology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The insulin-like androgenic gland (IAG) gene is well known in male crustacean, and it is a key regulator in male sexual differentiation and maintaining the male sexual characteristic. The neo-female can be produced by silencing the MrIAG (Macrobrachium rosenbergii Insulin-like Androgenic Gland) in male Macrobrachium rosenbergii. This is the first time to use siRNA approach to silenced MrIAG in male M. rosenbergii. In the current study, the optimal injection dosage to achieve sex reversal is 0.5 μg/g body weight. After MrIAG silencing, the expression level of Dmrt11e, Dmrt99b, MRPINK, Mrr, Sxl1, and Sxl2 decreased significantly. As their long-term silencing effect of MrIAG, the dsRNA and siRNA approaches produce three and two individual neo-females, respectively. The neo-female has a wider brood chamber, ovipositing setae, and ovigerous setae, which is resembled normal female. After a long-term silencing with siRNA, most of the germ cells were arrested in spermatocytes stage, but the spermatocytes in control can further developed into spermatozoon. The seminiferous tubules are loosely arranged and the spermatocytes are more than spermatozoon in the 0.5 μg/g body weight treatment dose. This current study suggests a new path to obtain neo-females through siRNA silencing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.