ArticleMetabolic engineering2020
Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers.
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Article in Metabolic engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40citing papers in PubMed
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Who cites it
40 citing papers in PubMed.
- Organosulfur compounds as dual-action agents: a critical review of antimicrobial and immunomodulatory potentials, and translational barriers.Frontiers in pharmacology · 2026Review
- Enhanced bioproduction and processing of mandelic acid enantiomers: towards a sustainable platform for high-value pharmaceutical and polymer applications.Biotechnology for biofuels and bioproducts · 2025Article
- Key Technologies of Synthetic Biology in Industrial Microbiology.Microorganisms · 2025Review
- Combinatorial engineering pinpoints shikimate pathway bottlenecks in para-aminobenzoic acid production in Pseudomonas putida.Journal of biological engineering · 2025Article
- Abstraction hierarchy to define biofoundry workflows and operations for interoperable synthetic biology research and applications.Nature communications · 2025Review
- Article
- Engineering an Escherichia coli strain for enhanced production of flavonoids derived from pinocembrin.Microbial cell factories · 2024Article
- Getting the Right Clones in an Automated Manner: An Alternative to Sophisticated Colony-Picking Robotics.Bioengineering (Basel, Switzerland) · 2024Article
- Artificial Intelligence Methods and Models for Retro-Biosynthesis: A Scoping Review.ACS synthetic biology · 2024Article
- Bioproduction of methylated phenylpropenes and isoeugenol inMetabolic engineering communications · 2024Article
- Genetically Engineered Microorganisms and Their Impact on Human Health.International journal of clinical practice · 2024Review
- Computer-aided design and implementation of efficient biosynthetic pathways to produce high added-value products derived from tyrosine inFrontiers in bioengineering and biotechnology · 2024Article
- Carboxylic acid reductase-dependent biosynthesis of eugenol and related allylphenols.Microbial cell factories · 2023Article
- Microbial production of the plant flavanone hesperetin from caffeic acid.BMC research notes · 2023Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- A tripartite microbial co-culture system for de novo biosynthesis of diverse plant phenylpropanoids.Nature communications · 2023Article
- Engineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system.Biotechnology for biofuels and bioproducts · 2023Article
- Sustainable biosynthetic pathways to value-added bioproducts from hydroxycinnamic acids.Applied microbiology and biotechnology · 2023Review
- TFBMiner: A User-Friendly Command Line Tool for the Rapid Mining of Transcription Factor-Based Biosensors.ACS synthetic biology · 2023Article
- Co-production of biofuel, bioplastics and biochemicals during extended fermentation of Halomonas bluephagenesis.Microbial biotechnology · 2023Article
Corrections and comments
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Authors and funding
23 authors.
Christopher J RobinsonManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Christopher.Robinson@manchester.ac.uk.
Pablo CarbonellManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: pablo.carbonell@manchester.ac.uk.
Adrian J JervisManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Adrian.Jervis@manchester.ac.uk.
Cunyu YanManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: cunyu.yan@manchester.ac.uk.
Katherine A HollywoodManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: katherine.hollywood@manchester.ac.uk.
Mark S DunstanManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Mark.Dunstan@manchester.ac.uk.
Andrew CurrinManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: andrew.currin@manchester.ac.uk.
Neil SwainstonManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Neil.Swainston@manchester.ac.uk.
Reynard SpiessManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Reynard.Spiess@manchester.ac.uk.
Sandra TaylorManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Sandra.Taylor@manchester.ac.uk.
Paul MulherinManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: paul.mulherin@manchester.ac.uk.
Steven ParkerManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: steven.parker@manchester.ac.uk.
William RoweManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: William.rowe@manchester.ac.uk.
Nicholas E MatthewsManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Manchester Institute of Innovation Research, Alliance Manchester Business School, The University of Manchester, Manchester, M15 6PB, UK. Electronic address: n.matthews@manchester.ac.uk.
Kirk J MaloneManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Kirk.Malone@manchester.ac.uk.
Rosalind Le FeuvreManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: r.a.le-feuvre@manchester.ac.uk.
Philip ShapiraManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Manchester Institute of Innovation Research, Alliance Manchester Business School, The University of Manchester, Manchester, M15 6PB, UK. Electronic address: PShapira@manchester.ac.uk.
Perdita BarranManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: perdita.barran@manchester.ac.uk.
Nicholas J TurnerManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Nicholas.Turner@manchester.ac.uk.
Jason MicklefieldManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: jason.micklefield@manchester.ac.uk.
Rainer BreitlingManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Rainer.Breitling@manchester.ac.uk.
Eriko TakanoManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Eriko.Takano@manchester.ac.uk.
Nigel S ScruttonManchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Nigel.Scrutton@manchester.ac.uk.
Funding
Biotechnology and Biological Sciences Research Council BB/M017702/1
Abstract
Bio-based production of industrial chemicals using synthetic biology can provide alternative green routes from renewable resources, allowing for cleaner production processes. To efficiently produce chemicals on-demand through microbial strain engineering, biomanufacturing foundries have developed automated pipelines that are largely compound agnostic in their time to delivery. Here we benchmark the capabilities of a biomanufacturing pipeline to enable rapid prototyping of microbial cell factories for the production of chemically diverse industrially relevant material building blocks. Over 85 days the pipeline was able to produce 17 potential material monomers and key intermediates by combining 160 genetic parts into 115 unique biosynthetic pathways. To explore the scale-up potential of our prototype production strains, we optimized the enantioselective production of mandelic acid and hydroxymandelic acid, achieving gram-scale production in fed-batch fermenters. The high success rate in the rapid design and prototyping of microbially-produced material building blocks reveals the potential role of biofoundries in leading the transition to sustainable materials production.
Indexed as
BacteriaBiosynthetic PathwaysChemical IndustryComputer SimulationFermentationIndustrial MicrobiologyMandelic AcidsMetabolic EngineeringStereoisomerismmandelic acidMandelic AcidsBiofoundryBiomanufacturingHydroxymandelic acidIndustrial biotechnologyMandelic acidSynthetic biology
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