Evidence map›Paper›PMID 32334529›Full record

ArticleBMC infectious diseases2020

Existence of hepatitis B virus surface protein mutations and other variants: demand for hepatitis B infection control in Cambodia.

Ko Ko, Kazuaki Takahashi, Shintaro Nagashima, Chikako Yamamoto, Vichit Ork, Aya Sugiyama, Tomoyuki Akita, Masayuki Ohisa, Channarena Chuon, Md Shafiqul Hossain and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Ko KoDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Kazuaki TakahashiDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Shintaro NagashimaDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Chikako YamamotoDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Vichit OrkNational Immunization Programme, Ministry of Health, 151-152 Kampuchea Krom Avenue, Phonm Penh, Cambodia.
Aya SugiyamaDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Tomoyuki AkitaDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Masayuki OhisaDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Channarena ChuonDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Md Shafiqul HossainExpanded Programme on Immunization, World Health Organization Country Office, Cambodia; 61-64, Preah Norodom Blvd (St 306), Sangkat Boeung Keng Kang I, Khan Chamkamom, Phnom Penh, Cambodia.
Bunsoth MaoUniversity of Health Sciences, 73 Monivong Boulevard, Phnom Penh, Cambodia.
Junko TanakaDepartment of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan; 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan. jun-tanaka@hiroshima-u.ac.jp.
Hiroshima University · JPMinistry of Health · KHUniversity of Health Science · KHWorld Health Organization - Pakistan · PK

Funding

Ministry of Health, Labour and Welfare, Japan H28-Kansei-Japan-001Project Research Centre for epidemiology and prevention of viral hepatitis and hepatocellular carcinoma, Hiroshima University, Japan NoneViral hepatitis Research Foundation of Japan (AAOHELP: Asia-Africa-Oceania Hepatitis Experts Linkage Promotion) NoneWorld Health Organization 001World Health Organization: The Expanded Programme on Immunization (EPI) None
6 · The paper itself

Abstract

backgroundThis study aimed to detect Hepatitis B virus (HBV) genome sequences and their variants as of nationwide scale using dried blood spot (DBS) samples and to provide up-to-date reference data for infection control and surveillance in Cambodia.

methodAmong 2518 children age 5-7 years and their 2023 mothers participated in 2017 Cambodia nationwide sero-survey on hepatitis B surface antigen (HBsAg) prevalence using multistage random sampling strategy, 95 mothers and 13 children positive to HBsAg were included in this study. HBV DNA was extracted from DBS, then performed polymerase chain reaction. HBV genotypes and potential variants were examined by partial and full length genomic analysis.

resultsHBsAg positive rate was 4.7% (95/2023) in mothers and 0.52% (13/2518) in their children. Genotype C (80.49%) was abundantly found throughout the whole Cambodia whilst genotype B (19.51%) was exclusively found in regions bordering Vietnam. S gene mutants of HBV were found in 24.29% of mothers and 16.67% of children with HBV DNA positive sera. Full-length genome analysis revealed the homology of 99.62-100% in each mother-child pair. Genotype B was clarified to recombinant genotype B4/C2 and B2/C2. Double (48.39%) and combination mutation (32.26%) were observed in core promoter region of HBV C1 strains.

conclusionsThis study showed the capable of DBS for large-scale molecular epidemiological study of HBV in resource limited countries. Full-genome sequences yield the better understanding of sub-genotypes, their variants and the degree of homology between strains isolated from mother-child pairs calls for effective strategies on prevention, control and surveillance of mother-to-child HBV transmission in Cambodia.

Indexed as

MutationAdultCambodiaChildChild, PreschoolDried Blood Spot TestingFemaleGenome, ViralGenotypeHepatitis BHepatitis B Surface AntigensHepatitis B virusHumansInfectious Disease Transmission, VerticalMothersPhylogenyHepatitis B Surface AntigensCambodiaDried blood spotFull genome sequenceGenotypeHepatitis B virusMutationNationwide

Identifiers

PMID32334529
PMCPMC7183719
OpenAlexW3018754948

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.