Evidence map›Paper›PMID 32334450›Full record

ArticleAsian Pacific journal of cancer prevention : APJCP2020

The Effect of PEI-Mediated E1A on the Radiosensitivity of Hepatic Carcinoma Cells.

Danghui Xu, Jianxin Yao, Yiwen Zhang, Nan Xiao, Peng Peng, Zhanfeng Li, Zhiyao Pan, Zhifeng Yao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Asian Pacific journal of cancer prevention : APJCP, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Danghui XuDepartment of Radiology，Affiliated Hospital of Nanjing University of Chinese Medicine，Jiangsu Provincial Hospital of Traditional Chinese Medicine，Nanjing, Jiangsu Province ,China.
Jianxin YaoDepartment of Medical Imaging, Nanjing Vocational Health College, Nanjing, Jiangsu Province, China.
Yiwen ZhangDepartment of Nursing, The Affiliated Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Nan XiaoDepartment of Medical Imaging, Nanjing Vocational Health College, Nanjing, Jiangsu Province, China.
Peng PengDepartment of Nursing, Nanjing Health College of Jiangsu Union Technical Institute, Nanjing, Jiangsu Province, China.
Zhanfeng LiDepartment of Medical Imaging, Nanjing Vocational Health College, Nanjing, Jiangsu Province, China.
Zhiyao PanDepartment of Basic Medical Science, Zhejiang University Medical College, Hangzhou, Zhejiang Province, China.
Zhifeng YaoDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
Nanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe study was undertaken to investigate the effects of polyethyleneimine (PEI)-mediated adenovirus 5 early region 1A (E1A) on radiosensitivity of human hepatic carcinoma cell in vitro and to disclosure the underlying mechanism. MATERIALS AND

methodsHuman hepatic carcinoma SMMC-7721 cell line was transfected with E1A gene using PEI vector. Untransfected cells (SMMC-7721 group), cells transfected with blank-vector (SMMC-7721-vect group), and cells transfected with E1A gene (SMMC-7721-E1A group) were treated with 6 MV X-ray irradiation at doses of 0, 1, 2, 4, 8 and Gy, respectively. Radiosensitivity was determined by MTT assay and quantified by calculating the cell survival rate. Cell-cycle distribution and apotosis rate were monitored by flow cytometry.

resultsThe survival rate of SMMC-7721-E1A was significantly lower than that of SMMC-7721 cell. Apoptosis rate of SMMC-7721-E1A group was significantly higher than that of SMMC-7721group (P<0.01).The ratio of S stage in cell cycle of SMMC-7721-E1A was significantly lower than that in SMMC-7721 cell. The ratio of G2/M stage in cell cycle of SMMC-7721-E1A was significantly higher than that in SMMC-7721 cell (P<0.01).

conclusionPEI could transfect E1A gene into hepatic carcinoma cells PEI-mediated E1A could effectively enhance radiosensitivity of hepatic carcinoma cells which may be related to its effects on apoptosis promoting leading to S phase suppression and G2/M phase arrest.<br />.

Indexed as

Adenovirus E1A ProteinsApoptosisCarcinoma, HepatocellularCell CycleCell ProliferationHumansLiver NeoplasmsPolyethyleneimineRadiation ToleranceTumor Cells, CulturedAdenovirus E1A ProteinsPolyethyleneimineadenovirus 5 early region 1AHuman hepatic carcinoma cellpolyethyleneimineradio-sensitivity

Identifiers

PMID32334450
PMCPMC7445989
OpenAlexW3020574321

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.