ArticlePlant cell reports2020
Expression of the subgroup IIIf bHLH transcription factor CpbHLH1 from Chimonanthus praecox (L.) in transgenic model plants inhibits anthocyanin accumulation.
Article in Plant cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 98 citations in OpenAlex.
- Article
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- Integrated Multiomics Analysis Sheds Light on the Mechanisms of Color and Fragrance Biosynthesis in Wintersweet Flowers.International journal of molecular sciences · 2025Article
- Comparative Transcriptomic Analyses of Anthocyanin Biosynthesis Genes in Eggplant Under Low Temperature and Weak Light.Plants (Basel, Switzerland) · 2025Article
- Comparative analysis of the WRKY gene family between Chimonanthus praecox and C. salicifolius.Genetica · 2025Article
- Interaction of PsMYB4 with PsEGL3 inhibits anthocyanin biosynthesis in tree peony yellow flowers.Frontiers in plant science · 2025Article
- Genome-wide identification and expression analysis of soybean bHLH transcription factor and its molecular mechanism on grain protein synthesis.Frontiers in plant science · 2025Article
- The Complex FtBBX22 and FtHY5 Positively Regulates Light-Induced Anthocyanin Accumulation by ActivatingInternational journal of molecular sciences · 2024Article
- Review
- Tartary Buckwheat (International journal of molecular sciences · 2023Article
- Genome-Wide Analysis of the bHLH Gene Family inPlants (Basel, Switzerland) · 2023Article
- A genome‑wide approach to the systematic and comprehensive analysis of LIM gene family in sorghum (Sorghum bicolor L.).Genomics & informatics · 2023Article
- Genome-Wide Identification and Expression Analysis of the bHLH Transcription Factor Family in Wintersweet (International journal of molecular sciences · 2023Article
- Independent flavonoid and anthocyanin biosynthesis in the flesh of a red-fleshed table grape revealed by metabolome and transcriptome co-analysis.BMC plant biology · 2023Article
- Yeast One-Hybrid Screening for Transcription Factors of IbbHLH2 in Purple-Fleshed Sweet Potato.Genes · 2023Article
- GhWRKY41 forms a positive feedback regulation loop and increases cotton defence response against Verticillium dahliae by regulating phenylpropanoid metabolism.Plant biotechnology journal · 2023Article
- RcbHLH59-RcPRs module enhances salinity stress tolerance by balancing NaHorticulture research · 2023Article
- Multi-omics analysis reveals the mechanism of bHLH130 responding to low-nitrogen stress of apple rootstock.Plant physiology · 2023Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
key messageOverexpression of CpbHLH1 in Arabidopsis and tobacco resulted in a dramatic decrease in anthocyanin accumulation by repressing the expression of late biosynthesis genes in the flavonoid biosynthesis pathway. Many basic helix-loop-helix (bHLH) transcription factors (TFs) of subgroup IIIf have been characterized as anthocyanin-associated activators in higher plants, but information regarding bHLH TFs that inhibit anthocyanin accumulation remains scarce. In this study, the subgroup IIIf bHLH TF CpbHLH1 from Chimonanthus praecox (L.) was identified as a negative regulator of anthocyanin accumulation. Our results showed that overexpression of CpbHLH1 in model plant species, Arabidopsis and tobacco, resulted in a dramatic decrease in anthocyanin content, whereas the content of proanthocyanidin was little affected. Quantitative RT-PCR (qRT-PCR) assays of the structural genes in the flavonoid biosynthesis pathway revealed that CpbHLH1 inhibits anthocyanin accumulation mainly through repressing the expression of late biosynthesis genes (LBGs). Interactions between CpbHLH1 protein and AtPAP1/NtAN2 protein were detected via yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. This is the first bHLH repressor of anthocyanin biosynthesis identified in dicotyledons. These results can help us better understand the anthocyanin regulatory network in plants and may provide insights into the diverse functions of bHLH proteins.
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