ArticleInternational journal of molecular sciences2020
Concentration Dependent Effect of Human Dermal Fibroblast Conditioned Medium (DFCM) from Three Various Origins on Keratinocytes Wound Healing.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Advancing wound healing by hydrogel-based dressings loaded with cell-conditioned medium: a systematic review.Stem cell research & therapy · 2024Pooled it
- Enhancing Wound Healing Through Secretome-Loaded 3D-Printed Biomaterials.Gels (Basel, Switzerland) · 2025Review
- Innovative Strategies: Use of Stromal Cell-Derived Secretome for Chronic Wound Therapy.International journal of molecular sciences · 2025Review
- Cost-reduction strategy to culture patient derived bladder tumor organoids.Scientific reports · 2025Article
- Optimisation of cryopreservation conditions, including storage duration and revival methods, for the viability of human primary cells.BMC molecular and cell biology · 2024Article
- Effect of Multiple-Cycle Collections of Conditioned Media from Different Cell Sources towards Fibroblasts in In Vitro Wound Healing Model.Pharmaceutics · 2024Article
- Proteomic Identification and Quantification of Secretory Proteins in Human Dermal Fibroblast-Conditioned Medium for Wound Repair and Hair Regeneration.Clinical, cosmetic and investigational dermatology · 2023Article
- Effect of Different Collection Times of Dermal Fibroblast Conditioned Medium (DFCM) on In Vitro Re-Epithelialisation Process.Biomedicines · 2022Article
- Hybrid Collagen Hydrogel/Chondroitin-4-Sulphate Fortified with Dermal Fibroblast Conditioned Medium for Skin Therapeutic Application.Polymers · 2021Article
- Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Fibroblasts secrete many essential factors that can be collected from fibroblast culture medium, which is termed dermal fibroblast conditioned medium (DFCM). Fibroblasts isolated from human skin samples were cultured in vitro using the serum-free keratinocyte-specific medium (Epilife (KM1), or define keratinocytes serum-free medium, DKSFM (KM2) and serum-free fibroblast-specific medium (FM) to collect DFCM-KM1, DFCM-KM2, and DFCM-FM, respectively). We characterised and evaluated the effects of 100-1600 µg/mL DFCM on keratinocytes based on attachment, proliferation, migration and gene expression. Supplementation with 200-400 µg/mL keratinocyte-specific DFCM-KM1 and DFCM-KM2 enhanced the attachment, proliferation and migration of sub-confluent keratinocytes, whereas 200-1600 µg/mL DFCM-FM significantly increased the healing rate in the wound healing assay, and 400-800 µg/mL DFCM-FM was suitable to enhance keratinocyte attachment and proliferation. A real-time (RT
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