ArticleVirus genes2020
Isolation and characterization of new Puumala orthohantavirus strains from Germany.
Article in Virus genes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Discovery and characterization of Sager Creek virus, a new orthohantavirus in prairie voles (Microtus ochrogaster).Npj viruses · 2025Article
- Isolation and characterization of genetic variants of Orthohantavirus hantanense from clinical cases of HFRS in Jiangxi Province, China.PLoS neglected tropical diseases · 2024Article
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- Tropism ofViruses · 2023Article
- Hemorrhagic Fever with Renal Syndrome: Literature Review, Epidemiology, Clinical Picture and Pathogenesis.Infection & chemotherapy · 2022Review
- Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France.Pathogens (Basel, Switzerland) · 2021Article
- Article
- Orthohantaviruses, Emerging Zoonotic Pathogens.Pathogens (Basel, Switzerland) · 2020Review
- Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment.Pathogens (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Orthohantaviruses are re-emerging rodent-borne pathogens distributed all over the world. Here, we report the isolation of a Puumala orthohantavirus (PUUV) strain from bank voles caught in a highly endemic region around the city Osnabrück, north-west Germany. Coding and non-coding sequences of all three segments (S, M, and L) were determined from original lung tissue, after isolation and after additional passaging in VeroE6 cells and a bank vole-derived kidney cell line. Different single amino acid substitutions were observed in the RNA-dependent RNA polymerase (RdRP) of the two stable PUUV isolates. The PUUV strain from VeroE6 cells showed a lower titer when propagated on bank vole cells compared to VeroE6 cells. Additionally, glycoprotein precursor (GPC)-derived virus-like particles of a German PUUV sequence allowed the generation of monoclonal antibodies that allowed the reliable detection of the isolated PUUV strain in the immunofluorescence assay. In conclusion, this is the first isolation of a PUUV strain from Central Europe and the generation of glycoprotein-specific monoclonal antibodies for this PUUV isolate. The obtained virus isolate and GPC-specific antibodies are instrumental tools for future reservoir host studies.
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