Evidence map›Paper›PMID 32328815›Full record

ArticleWorld journal of microbiology & biotechnology2020

Autolysis, plasmolysis and enzymatic hydrolysis of baker's yeast (Saccharomyces cerevisiae): a comparative study.

Zeinab Takalloo, Mohsen Nikkhah, Robabeh Nemati, Nezam Jalilian, Reza H Sajedi

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
4.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 110 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Microencapsulation ofFrontiers in microbiology · 2026
    Article
  8. Review
  9. Using autolyzed and driedFood chemistry: X · 2025
    Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Review
  15. Microencapsulation of Spent Coffee Extract WithinFoods (Basel, Switzerland) · 2025
    Article
  16. Frontiers in nutrition · 2025
    Article
  17. Review
  18. Article
  19. Spent Brewer's Yeast Lysis Enables aInternational journal of molecular sciences · 2024
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Zeinab TakallooKimiazyme Company, Modares Science & Technology Park, Tarbiat Modares University, Tehran, Iran.
Mohsen NikkhahKimiazyme Company, Modares Science & Technology Park, Tarbiat Modares University, Tehran, Iran.
Robabeh NematiKimiazyme Company, Modares Science & Technology Park, Tarbiat Modares University, Tehran, Iran.
Nezam JalilianDepartment of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-154, Iran.
Reza H SajediKimiazyme Company, Modares Science & Technology Park, Tarbiat Modares University, Tehran, Iran. sajedi_r@modares.ac.ir.
Tarbiat Modares University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Saccharomyces cerevisiae is being used for long as a rich source of proteins, sugars, nucleotides, vitamins and minerals. Autolyzed and hydrolyzed yeast biomass has found numerous applications in the health food industry as well as livestock feeds. Here, we have compared three lysis methods for production of yeast lysates using autolysis, plasmolysis (ethyl acetate 1.5%), and enzymatic hydrolysis (Alcalase 0.2%). The efficiency of each process was compared according to soluble solid and protein contents, cell lysis monitoring, and release of intracellular materials, cell viability and microscopic analysis. Results showed that plasmolysis by ethyl acetate was found to be more efficient compared to autolysis, with a higher recovery of yeast extract (YE) content. In comparison, the content of released solids and proteins were higher during the enzymatic hydrolysis using Alcalase compared to autolysis and plasmolysis treatments. The highest decrease in optical density of 600 nm was monitored for the hydrolyzed cells. Besides, we defined "Degree of Leakage (DL)" as a new index of the lysis process, referring to the percentage of total released proteins from the cells and it was estimated to about 65.8%, which represents an appropriate indicator of the cell lysis. The biochemical and biophysical properties of the hydrolyzed yeast product as well as its biological activity (free radical scavenging activity and bacterial binding capacity) suggest that Alcalase could be used to accelerate the lysis of yeast cells and release the valuable intracellular components used for foodstuffs, feed and fermentation media applications. Production of baker's yeast lysates using autolysis, plasmolysis, and enzymatic hydrolysis methods.

Indexed as

AutolysisHydrolysisAcetatesBiomassCulture MediaFermentationIndustrial MicrobiologySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAcetatesCulture Mediaethyl acetateSaccharomyces cerevisiae ProteinsAutolyzed yeastDegree of LeakageEnzymatic hydrolysisPlasmolysisSaccharomyces cerevisiae

Identifiers

PMID32328815
OpenAlexW3019594745

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.