Evidence map›Paper›PMID 32328641›Full record

ArticleThe Journal of cell biology2020

A direct role for SNX9 in the biogenesis of filopodia.

Iris K Jarsch, Jonathan R Gadsby, Annalisa Nuccitelli, Julia Mason, Hanae Shimo, Ludovic Pilloux, Bishara Marzook, Claire M Mulvey, Ulrich Dobramysl, Charles R Bradshaw and 5 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Frontiers in cellular and infection microbiology · 2023
    Article
  7. Review
  8. Article
  9. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Iris K JarschGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
Jonathan R GadsbyGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
Annalisa NuccitelliAntibody Discovery and Protein Engineering, AstraZeneca, Granta Park, Cambridge, UK.
Julia MasonGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
Hanae ShimoGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
Ludovic PillouxDepartment of Pathology, University of Cambridge, Cambridge, UK.
Bishara MarzookGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
Claire M MulveyGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
Ulrich DobramyslGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
Charles R BradshawGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
Kathryn S LilleyDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Richard D HaywardDepartment of Pathology, University of Cambridge, Cambridge, UK.
Tristan J VaughanAntibody Discovery and Protein Engineering, AstraZeneca, Granta Park, Cambridge, UK.
Claire L DobsonAntibody Discovery and Protein Engineering, AstraZeneca, Granta Park, Cambridge, UK.
Jennifer L GallopGurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, UK.
University of Cambridge · GBAstraZeneca (United Kingdom) · GB

Funding

Cancer Research UK C6946/A14492European Research Council 281971Medical Research Council MR/T030089/1Medical Research Council N000846/1Wellcome Trust 092096Wellcome Trust 105602/Z/14/ZWellcome Trust 108467/Z/15/ZWellcome Trust WT095829AIA
6 · The paper itself

Abstract

Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell-cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia.

Indexed as

AnimalsFemaleHeLa CellsHumansMalePseudopodiaSorting NexinsXenopus laevisXenopus ProteinsSNX9 protein, XenopusSorting NexinsXenopus Proteins

Identifiers

PMID32328641
PMCPMC7147113
OpenAlexW3013042132

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.