ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2020
Dissolvable Polyacrylamide Beads for High-Throughput Droplet DNA Barcoding.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- Evaluating single-cell ATAC-seq atlasing technologies using sequence-to-function modeling.Nature communications · 2026Article
- A droplet microfluidics-based platform for generating target-specific, natively-paired immune libraries and identifying potent and developable antibodies.Scientific reports · 2026Article
- Single-Cell Nucleus Extraction with Cellular Indexing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Single-cell sequencing technology in renal cancer: insights into tumor biology and clinical application.Biomarker research · 2025Review
- High-throughput single-microbe RNA sequencing reveals adaptive state heterogeneity and host-phage activity associations in human gut microbiome.Protein & cell · 2025Article
- Liquid-Solid Triboelectric Nanogenerator-Based DNA Barcode Detection Biosensor for Species Identification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- High-Throughput Single-Nucleus RNA Profiling of Minimal Puncture FFPE Samples Reveals Spatiotemporal Heterogeneity of Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Hydrogel bead-based isothermal detection (BEAD-ID) for assessing the activity of DNA-modifying enzymes.iScience · 2024Article
- A Versatile Microchannel Array Device for Portable and Parallel Droplet Generation.Small science · 2024Article
- Processing DNA Storage through Programmable Assembly in a Droplet-Based Fluidics System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Microbiome single cell atlases generated with a commercial instrument.Research square · 2023Article
- Droplet-based high-throughput single microbe RNA sequencing by smRandom-seq.Nature communications · 2023Article
- High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seq.Nature communications · 2023Article
- Droplet Microfluidics Enables Tracing of Target Cells at the Single-Cell Transcriptome Resolution.Bioengineering (Basel, Switzerland) · 2022Article
- Highly sensitive single-cell chromatin accessibility assay and transcriptome coassay with METATAC.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Hydrop enables droplet-based single-cell ATAC-seq and single-cell RNA-seq using dissolvable hydrogel beads.eLife · 2022Article
- Simple One-Step and Rapid Patterning of PDMS Microfluidic Device Wettability for PDMS Shell Production.Frontiers in bioengineering and biotechnology · 2022Article
- Modular barcode beads for microfluidic single cell genomics.Scientific reports · 2021Article
- Article
- Dissolvable Polyacrylamide Beads for High-Throughput Droplet DNA Barcoding.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
Droplet-based single cell sequencing technologies, such as inDrop, Drop-seq, and 10X Genomics, are catalyzing a revolution in the understanding of biology. Barcoding beads are key components for these technologies. What is limiting today are barcoding beads that are easy to fabricate, can efficiently deliver primers into drops, and thus achieve high detection efficiency. Here, this work reports an approach to fabricate dissolvable polyacrylamide beads, by crosslinking acrylamide with disulfide bridges that can be cleaved with dithiothreitol. The beads can be rapidly dissolved in drops and release DNA barcode primers. The dissolvable beads are easy to synthesize, and the primer cost for the beads is significantly lower than that for the previous barcoding beads. Furthermore, the dissolvable beads can be loaded into drops with >95% loading efficiency of a single bead per drop and the dissolution of beads does not influence reverse transcription or the polymerase chain reaction (PCR) in drops. Based on this approach, the dissolvable beads are used for single cell RNA and protein analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.