ArticleNature communications2020
Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 266 citations in OpenAlex.
- Research progress of Rumen microbes utilizing lignocellulose for high-value chemical production.Biodesign research · 2026Review
- Compositionally Biased Regions Within Structured Protein Domains.Biomolecules · 2026Article
- Photoelectrocatalytic-microbial biohybrid for succinic acid synthesis.Nature communications · 2026Article
- Succinic Acid Production by Engineered Mannheimia succiniciproducens and Its Use in Chemoenzymatic Poly(Butylene Succinate) Synthesis.Biotechnology and bioengineering · 2025Article
- Construction and Characterization of Novel Shuttle Expression Vectors for Actinobacillus succinogenes.Biotechnology and applied biochemistry · 2025Article
- EngineeringJournal of microbiology and biotechnology · 2025Article
- Computational scoring and experimental evaluation of enzymes generated by neural networks.Nature biotechnology · 2025Article
- Advancements in metabolic engineering: unlocking the potential of key organic acids for sustainable industrial applications.Frontiers in bioengineering and biotechnology · 2025Review
- Production of Succinic Acid by Metabolically EngineeredJournal of microbiology and biotechnology · 2024Article
- Integrative analysis of toxicometabolomics and toxicoproteomics data: new molecular insights into thiazolidinedione-induced cardiotoxicity.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1.Microbial cell factories · 2024Article
- High-level succinic acid production by overexpressing a magnesium transporter inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Achieving robust synthetic tolerance in industrialSynthetic and systems biotechnology · 2024Article
- Review
- Recent advances in bio-based production of top platform chemical, succinic acid: an alternative to conventional chemistry.Biotechnology for biofuels and bioproducts · 2024Review
- Advances in succinic acid production: the enhancement of COFrontiers in bioengineering and biotechnology · 2024Review
- Development of highly efficient and specific base editors in Actinobacillus succinogenes for enhancing succinic acid production.Biotechnology for biofuels and bioproducts · 2023Article
- Sustainable production and degradation of plastics using microbes.Nature microbiology · 2023Review
- Biotechnological Plastic Degradation and Valorization Using Systems Metabolic Engineering.International journal of molecular sciences · 2023Review
- An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis.Nature communications · 2023Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Succinic acid (SA), a dicarboxylic acid of industrial importance, can be efficiently produced by metabolically engineered Mannheimia succiniciproducens. Malate dehydrogenase (MDH) is one of the key enzymes for SA production, but has not been well characterized. Here we report biochemical and structural analyses of various MDHs and development of hyper-SA producing M. succiniciproducens by introducing the best MDH. Corynebacterium glutamicum MDH (CgMDH) shows the highest specific activity and least substrate inhibition, whereas M. succiniciproducens MDH (MsMDH) shows low specific activity at physiological pH and strong uncompetitive inhibition toward oxaloacetate (ki of 67.4 and 588.9 μM for MsMDH and CgMDH, respectively). Structural comparison of the two MDHs reveals a key residue influencing the specific activity and susceptibility to substrate inhibition. A high-inoculum fed-batch fermentation of the final strain expressing cgmdh produces 134.25 g L
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.