ArticleBMC genomics2020
Transcriptome analysis reveals differential immune related genes expression in Ruditapes philippinarum under hypoxia stress: potential HIF and NF-κB crosstalk in immune responses in clam.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 83 citations in OpenAlex.
- Synergistic Effects of Ammonia and Hypoxia Stress on the Transcriptomic Responses of the Razor Clam (Animals : an open access journal from MDPI · 2026Article
- Metabolic and Biochemical Responses of JuvenileBiology · 2025Article
- Enhancement of Hypoxia Tolerance of Gibel Carp (Antioxidants (Basel, Switzerland) · 2025Article
- Transcriptomic Responses of Blue Bat StarInternational journal of molecular sciences · 2025Article
- Antioxidant Capacity, Enzyme Activities Related to Energy Metabolism, and Transcriptome Analysis ofAntioxidants (Basel, Switzerland) · 2024Article
- Hypoxia in the Blue MusselGenes · 2024Article
- Transcriptome analysis reveals regulatory mechanism of methyl jasmonate-induced monoterpenoid biosynthesis inFrontiers in plant science · 2024Article
- Liver Transcriptome Shows Differences between Acute Hypoxia-Tolerant and Intolerant Individuals of Greater Amberjack (Animals : an open access journal from MDPI · 2023Article
- Role of the Neuroendocrine System of Marine Bivalves in Their Response to Hypoxia.International journal of molecular sciences · 2023Review
- Evidence of multiple genome duplication events in Mytilus evolution.BMC genomics · 2022Article
- Integrated Metabolomics and Transcriptomic Analysis of Hepatopancreas in Different Living StatusAntioxidants (Basel, Switzerland) · 2021Article
- Transcriptomic analysis provides insights into candidate genes and molecular pathways involved in growth of Manila clam Ruditapes philippinarum.Functional & integrative genomics · 2021Article
- RNA-Sequencing Analysis of the Spleen and Gill ofFrontiers in veterinary science · 2021Article
- New insights into the Manila clam and PAMPs interaction based on RNA-seq analysis of clam through in vitro challenges with LPS, PGN, and poly(I:C).BMC genomics · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundHypoxia is an important environmental stressor in aquatic ecosystems, with increasingly impacts on global biodiversity. Benthic communities are the most sensitive parts of the coastal ecosystem to eutrophication and resulting hypoxia. As a filter-feeding organism living in the seafloor sediment, Ruditapes philippinarum represents an excellent "sentinel" species to assess the quality of marine environment. In order to gain insight into the molecular response and acclimatization mechanisms to hypoxia stress in marine invertebrates, we examined hypoxia-induced changes in immune-related gene expression and gene pathways involved in hypoxia regulation of R. philippinarum.
resultsWe investigated the response of the Manila clam R. philippinarum to hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific genes associated with hypoxia response by RNA-seq and time course qPCR analysis. A total of 75 genes were captured significantly differentially expressed, and were categorized into antioxidant/oxidative stress response, chaperones/heat shock proteins, immune alteration, and cell proliferation/apoptosis. Fourteen hypoxia responsive genes were validated significantly up/down regulated at different time 0, 2, 5, and 8 d in gills of R. philippinarum in hypoxia challenged group. Functional enrichment analysis revealed the HIF signaling pathway and NF-κB signaling pathway play pivotal roles in hypoxia tolerance and resistance in R. philippinarum.
conclusionThe HIF signaling pathway and NF-κB signaling pathway play a critical role in hypoxia tolerance and resistance in Manila clam. The immune and defense related genes and pathways obtained here gain a fundamental understanding of the hypoxia stress in marine bivalves and provide important insights into the physiological acclimation, immune response and defense activity under hypoxia challenge. The reduced metabolism is a consequence of counterbalancing investments in immune defense against other physiological processes.
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