Evidence map›Paper›PMID 32326440›Full record

ArticleCells2020

The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation.

Shantae M Thornton, Varsha D Samararatne, Joseph G Skeate, Christopher Buser, Kim P Lühen, Julia R Taylor, Diane M Da Silva, W Martin Kast

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. CD207 Expression Level is a New Prognostic Marker for Condyloma Acuminatum.Clinical, cosmetic and investigational dermatology · 2023
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Shantae M ThorntonDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USA.
Varsha D SamararatneDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USA.
Joseph G SkeateDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USA.ORCID 0000-0002-1765-5949
Christopher BuserOak Crest Institute of Science, Monrovia, CA 91016, USA.
Kim P LühenNorris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA.
Julia R TaylorDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USA.
Diane M Da SilvaNorris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA.
W Martin KastDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USA.ORCID 0000-0003-2321-3159
University of Southern California · USOak Crest Institute of Science · US

Funding

USC/NORRIS COMPREHENSIVE CANCER CENTER (CORE) SUPPORTP30CA014089 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI William Dean Wallace · 1985 to 2026
$181.4M
VLP VACCINES AGAINST HPV INDUCED TUMORSR01CA074397 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KAST, WIJBE MARTIN · 1997 to 2020
$5.5M
Contributions of the Annexin A2 Heterotetramer to HPV16 EndocytosisF31AI136312 · NIAID · UNIVERSITY OF SOUTHERN CALIFORNIA · PI TAYLOR, JULIA ROSE · 2018 to 2018
$28k
NCI NIH HHS P30 CA014089NCI NIH HHS R01 CA074397NIAID NIH HHS F31 AI136312
6 · The paper itself

Abstract

Langerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studies of BG have historically focused on morphological characterizations, but BG have also been implicated in viral antigen processing which suggests that they can serve a function in antiviral immunity. This study focused on investigating proteins that could be involved in BG formation to further characterize their structure using transmission electron microscopy (TEM). Here, we report a critical role for the protein annexin A2 (anxA2) in the proper formation of BG structures. When anxA2 expression is downregulated, langerin expression decreases, cytoplasmic BG are nearly ablated, and the presence of malformed BG-like structures increases. Furthermore, in the absence of anxA2, we found langerin was no longer localized to BG or BG-like structures. Taken together, these results indicate an essential role for anxA2 in facilitating the proper formation of BG.

Indexed as

Annexin A2Antigens, CDCell LineCytoplasmic GranulesHumansLangerhans CellsLectins, C-TypeMannose-Binding LectinsProtein SubunitsProtein TransportAnnexin A2Antigens, CDCD207 protein, humanLectins, C-TypeMannose-Binding LectinsProtein SubunitsA2tanxA2Birbeck granulesLangerhans cell

Identifiers

PMID32326440
PMCPMC7227008
OpenAlexW3016567458

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.