ArticleMicromachines2020
Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene.
Article in Micromachines, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- Cost-Effective Droplet Generator for Portable Bio-Applications.Micromachines · 2023Article
- Compact Camera Fluorescence Detector for Parallel-Light Lens-Based Real-Time PCR System.Sensors (Basel, Switzerland) · 2022Article
- Development of a low-cost multi-channel nucleic acid detection PCR instrument and clinical detection application of COVID-19.Analytica chimica acta · 2022Article
- Hundreds-Dollar-Level Multiplex Integrated RT-qPCR Quantitative System for Field Detection.Biosensors · 2022Article
- Low-Cost, Real-Time Polymerase Chain Reaction System for Point-of-Care Medical Diagnosis.Sensors (Basel, Switzerland) · 2022Article
- Cloud-Based Software Architecture for Fully Automated Point-of-Care Molecular Diagnostic Device.Sensors (Basel, Switzerland) · 2021Article
- Cost-Effective Multiplex Fluorescence Detection System for PCR Chip.Sensors (Basel, Switzerland) · 2021Article
- Fraud in Animal Origin Food Products: Advances in Emerging Spectroscopic Detection Methods over the Past Five Years.Foods (Basel, Switzerland) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the surface, and a new injection method solves the issue of bubbles formed during the temperature cycle. We built a battery-powered real-time PCR device to follow polymerase chain reaction using fluorescence detection and developed an independently designed electromechanical control system and a fluorescence analysis software to control the temperature cycle, the photoelectric detection coupling, and the automatic analysis of the experimental data. The microchips and the temperature cycling system cost USD 100. All the elements of the device are available through open access, and there are no technical barriers. The simple structure and manipulation allows beginners to build instruments and perform PCR tests after only a short tutorial. The device is used for analysis of the amplification curve and the melting curve of multiple target genes to demonstrate that our instrument has the same accuracy and stability as a commercial instrument.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.