ArticleNucleic acids research2020
Methyltransferase DnmA is responsible for genome-wide N6-methyladenosine modifications at non-palindromic recognition sites in Bacillus subtilis.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Type I restriction-modification systems regulate gene expression through DNA topology.Nucleic acids research · 2026Article
- Mapping the rRNA methylome reveals contributions of methyltransferases to ribosome function and antibiotic sensitivity.bioRxiv : the preprint server for biology · 2026Article
- Epigenomics identifies three sources of DNA methylation inbioRxiv : the preprint server for biology · 2026Article
- Epigenetic regulation in prokaryotes: transcriptional and phenotypic outcomes of DNA methyltransferase activity.FEMS microbiology reviews · 2026Review
- Epigenetic background of lineage-specific gene expression landscapes of four Staphylococcus aureus hospital isolates.PloS one · 2025Article
- BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus.Microbial cell factories · 2024Article
- Two distinct regulatory systems control pulcherrimin biosynthesis in Bacillus subtilis.PLoS genetics · 2024Article
- Characterization of a Novel N4-Methylcytosine Restriction-Modification System inInternational journal of molecular sciences · 2024Article
- Two Distinct Regulatory Systems Control Pulcherrimin Biosynthesis inbioRxiv : the preprint server for biology · 2024Article
- MLACNN: an attention mechanism-based CNN architecture for predicting genome-wide DNA methylation.Theory in biosciences = Theorie in den Biowissenschaften · 2023Article
- Article
- Protoplast fusion in Bacillus species produces frequent, unbiased, genome-wide homologous recombination.Nucleic acids research · 2022Article
- Detecting DNA Methylations in the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius Using SMRT Sequencing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Enzymatic deamination of the epigenetic nucleoside N6-methyladenosine regulates gene expression.Nucleic acids research · 2021Article
- DNA methylation by three Type I restriction modification systems of Escherichia coli does not influence gene regulation of the host bacterium.Nucleic acids research · 2021Article
- Clostridioides difficile specific DNA adenine methyltransferase CamA squeezes and flips adenine out of DNA helix.Nature communications · 2021Article
- Embryo-Like Features in Developing Bacillus subtilis Biofilms.Molecular biology and evolution · 2021Article
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7 authors.
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Abstract
The genomes of organisms from all three domains of life harbor endogenous base modifications in the form of DNA methylation. In bacterial genomes, methylation occurs on adenosine and cytidine residues to include N6-methyladenine (m6A), 5-methylcytosine (m5C), and N4-methylcytosine (m4C). Bacterial DNA methylation has been well characterized in the context of restriction-modification (RM) systems, where methylation regulates DNA incision by the cognate restriction endonuclease. Relative to RM systems less is known about how m6A contributes to the epigenetic regulation of cellular functions in Gram-positive bacteria. Here, we characterize site-specific m6A modifications in the non-palindromic sequence GACGmAG within the genomes of Bacillus subtilis strains. We demonstrate that the yeeA gene is a methyltransferase responsible for the presence of m6A modifications. We show that methylation from YeeA does not function to limit DNA uptake during natural transformation. Instead, we identify a subset of promoters that contain the methylation consensus sequence and show that loss of methylation within promoter regions causes a decrease in reporter expression. Further, we identify a transcriptional repressor that preferentially binds an unmethylated promoter used in the reporter assays. With these results we suggest that m6A modifications in B. subtilis function to promote gene expression.
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