ArticleJournal of biomedical materials research. Part A2020
Creation of a contractile biomaterial from a decellularized spinach leaf without ECM protein coating: An in vitro study.
Article in Journal of biomedical materials research. Part A, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Growing Relevance of Decellularized Plant Material as Functional Scaffolds in Tissue Engineering and Cultured Meat: A Scoping Review on Recent Advances, Challenges, and Future Directions.Annals of biomedical engineering · 2026Review
- Cultured Meat: A Multidimensional Review of Technological, Nutritional, Ethical, and Regulatory Advances (2020-2025).Journal of food science · 2026Review
- Plant-Based Scaffolds for Tissue Engineering: A Review.Polymers · 2025Review
- Decellularized Green and Brown Macroalgae as Cellulose Matrices for Tissue Engineering.Journal of functional biomaterials · 2024Article
- From Nature to Technology: Exploring the Potential of Plant-Based Materials and Modified Plants in Biomimetics, Bionics, and Green Innovations.Biomimetics (Basel, Switzerland) · 2024Review
- Advanced Cardiac Patches for the Treatment of Myocardial Infarction.Circulation · 2024Review
- Plant-Based Decellularization: A Novel Approach for Perfusion-Compatible Tissue Engineering Structures.Journal of microbiology and biotechnology · 2024Review
- Plant Decellularization by Chemical and Physical Methods for Regenerative Medicine: A Review Article.Journal of medical signals and sensors · 2024Review
- Plant Cellulose as a Substrate for 3D Neural Stem Cell Culture.Bioengineering (Basel, Switzerland) · 2023Article
- Aligned skeletal muscle assembly on a biofunctionalized plant leaf scaffold.Acta biomaterialia · 2023Article
- Small-Caliber Vascular Grafts Engineered from Decellularized Leaves and Cross-Linked Gelatin.Tissue engineering. Part A · 2023Article
- Recent Advances in Development of Natural Cellulosic Non-Woven Scaffolds for Tissue Engineering.Polymers · 2022Review
- Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Decellularization Strategies for Regenerating Cardiac and Skeletal Muscle Tissues.Frontiers in bioengineering and biotechnology · 2022Review
- The Emerging Role of Decellularized Plant-Based Scaffolds as a New Biomaterial.International journal of molecular sciences · 2021Review
- Cardiac mechanostructure: Using mechanics and anisotropy as inspiration for developing epicardial therapies in treating myocardial infarction.Bioactive materials · 2021Review
- Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds.Scientific reports · 2021Article
- A Novel Plant Leaf Patch Absorbed With IL-33 Antibody Decreases Venous Neointimal hyperplasia.Frontiers in bioengineering and biotechnology · 2021Article
- Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Myocardial infarction (MI) results in the death of cardiac tissue, decreases regional contraction, and can lead to heart failure. Tissue engineered cardiac patches containing human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) can restore contractile function. However, cells within thick patches require vasculature for blood flow. Recently, we demonstrated fibronectin coated decellularized leaves provide a suitable scaffold for hiPS-CMs. Yet, the necessity of this additional coating step is unclear. Therefore, we compared hiPS-CM behavior on decellularized leaves coated with collagen IV or fibronectin extracellular matrix (ECM) proteins to noncoated leaves for up to 21 days. Successful coating was verified by immunofluorescence. Similar numbers of hiPS-CMs adhered to coated and noncoated decellularized leaves for 21 days. At Day 14, collagen IV coated leaves contracted more than noncoated leaves (3.25 ± 0.39% vs. 1.54 ± 0.60%; p < .05). However, no differences in contraction were found between coated leaves, coated tissue culture plastic (TCP), noncoated leaves, or noncoated TCP at other time points. No significant differences were observed in hiPS-CM spreading or sarcomere lengths on leaves with or without coating. This study demonstrates that cardiac scaffolds can be created from decellularized leaves without ECM coatings. Noncoated decellularized leaf surfaces facilitate robust cell attachment for an engineered tissue patch.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.