Evidence map›Paper›PMID 32322610›Full record

ArticleData in brief2020

High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells.

Hanieh Mazloom-Farsibaf, William K Kanagy, Diane S Lidke, Keith A Lidke

Open access · goldAbstract read
In one paragraph

Article in Data in brief, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Hanieh Mazloom-FarsibafDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM, United States.
William K KanagyDepartment of Pathology, University of New Mexico, Albuquerque, NM, United States.
Diane S LidkeDepartment of Pathology, University of New Mexico, Albuquerque, NM, United States.
Keith A LidkeDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM, United States.
University of New Mexico · US

Funding

WOMEN'S CANCERS RESEARCH PROGRAMP30CA118100 · NCI · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Yolanda Sanchez · 2005 to 2026
$57.1M
UNM TRAININGP50GM085273 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI EDWARDS, JEREMY S · 2009 to 2018
$26.1M
Imaging the Early Events in Membrane Receptor SignalingR35GM126934 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Diane Lidke · 2018 to 2026
$3.1M
Structural and Functional Interactions within the Neuronal ER/PM JunctionR01GM109888 · NIGMS · COLORADO STATE UNIVERSITY · PI BEAM, KURT G, KRAFT, MARY L · 2014 to 2017
$1.5M
NCI NIH HHS P30 CA118100NIGMS NIH HHS P50 GM085273NIGMS NIH HHS R01 GM109888NIGMS NIH HHS R35 GM126934
6 · The paper itself

Abstract

A high-speed fluorescence microscope operating at a 490 Hz frame rate was used to image two different membrane proteins- the high-affinity IgE receptor FcɛRI, a transmembrane protein, and an outer-leaflet GPI-anchored protein. The IgE receptor was imaged via IgE labeled with Janelia Fluor 646 and the GPI-anchored protein was imaged using a GPI-GFP fusion protein and an ATTO 647 N labeled anti-GFP nanobody. Data was collected for both proteins in untreated cells and cells that had actin stabilized by phalloidin. This dataset can be used for development and testing of single-particle tracking methods on experimental data and to explore the hypothesis that the actin cytoskeleton may affect the movement of membrane proteins.

Indexed as

ActinFluorescence microscopyMembrane proteinsSingle-particle tracking

Identifiers

PMID32322610
PMCPMC7168344
OpenAlexW3015827885

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.