Evidence map›Paper›PMID 32322376›Full record

ArticleIranian journal of microbiology2020

Detection and characterization of purified antigenic proteins from culture filtrate of

Malihe Honarmand Kashi, Nader Mosavari, Mitra Salehi, Naheed Mojgani

Open access · diamondAbstract read
PubMed Full text at PMC
In one paragraph

Article in Iranian journal of microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.0field-weighted citation impact, top 62% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Malihe Honarmand KashiDepartment of Biology, Islamic Azad University of North Tehran Branch, Tehran, Iran.
Nader MosavariDepartment of PPD Tuberculin, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization, Karaj, Iran.
Mitra SalehiDepartment of Biology, Islamic Azad University of North Tehran Branch, Tehran, Iran.
Naheed MojganiDepartment of PPD Tuberculin, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization, Karaj, Iran.
Islamic Azad University North Tehran Branch · IRRazi Vaccine and Serum Research Institute · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesBovine tuberculosis diagnosis is usually performed by various tests with specific limitations. MATERIALS AND

methodsSecreted proteins were purified from the heat-treated culture filtrate of

resultsThe quantity of antigenic proteins in the culture medium was measured at far more than 15% of the amount of proteins secreted into medium. Three main chromatographic fractions obtained and showed concentrations of proteins ranging from 14 to 60 μg/ μl with molecular weights in the 10 to 180 kDa range. The purified antigens showed positive reactions to the infected cattle serum throughout dot-blotting. Western blotting revealed a total of 15 to 70 kDa molecular weight proteins.

conclusionImmunoblotting analysis made it possible to detect and recognize novel antigens that are useful for bovine tuberculosis diagnosis improvement. This is significant since non-specific reactions were not observed when we utilized serum of cattle experimentally infected with

Indexed as

Dot-blotMycobacterium bovisPurified antigenic proteinsWestern blot

Identifiers

PMID32322376
PMCPMC7163036
OpenAlexW3012472605

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.