ArticleAmerican journal of physiology. Lung cellular and molecular physiology2020
The importance of reporting house dust mite endotoxin abundance: impact on the lung transcriptome.
Article in American journal of physiology. Lung cellular and molecular physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Transcriptional regulation of the main olfactory epithelium by environmental olfactory exposures.Scientific reports · 2026Article
- Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics.Respiratory research · 2026Article
- Transcriptional regulation of the main olfactory epithelium by environmental olfactory exposures.bioRxiv : the preprint server for biology · 2026Article
- Epithelial Memory After Respiratory Viral Infection in Mice Results in Prolonged Enhancement of Antigen Presentation.Allergy · 2025Article
- LL-37 and citrullinated-LL-37 modulate IL-17A/F-mediated responses and selectively suppress Lipocalin-2 in bronchial epithelial cells.Journal of inflammation (London, England) · 2025Article
- Single-cell transcriptomics reveals immune remodeling of the murine lung microenvironment following chronic house dust mite exposure.Frontiers in immunology · 2025Article
- Article
- The implication of dendritic cells in lung diseases: Immunological role of toll-like receptor 4.Genes & diseases · 2024Review
- Article
- Adjuvant-independent airway sensitization and infection mouse models leading to allergic asthma.Frontiers in allergy · 2024Review
- Airway and parenchyma transcriptomics in a house dust mite model of experimental asthma.Respiratory research · 2023Article
- Investigating the microbiome of house dust mites in South Korea.Frontiers in allergy · 2023Review
- Production ofJournal of immunology research · 2023Article
- Characterization of sex-related differences in allergen house dust mite-challenged airway inflammation, in two different strains of mice.Scientific reports · 2022Article
- Comparative analysis of global transcriptome, proteome and acetylome in house dust mite-induced murine allergic asthma model.Clinical and translational medicine · 2021Article
- Assessment of airborne endotoxin in sandstorm dust and indoor environments using a novel passive sampling device in Al Zulfi city, Saudi Arabia - Establishing threshold exposure levels.Saudi journal of biological sciences · 2021Article
- Integrating Proteomes for Lung Tissues and Lavage Reveals Pathways That Link Responses in Allergen-Challenged Mice.ACS omega · 2021Article
- Systematic Characterization of the Group 2 House Dust Mite Allergen inFrontiers in cellular and infection microbiology · 2021Article
- Comparison of Allergenic Properties among Commercially Available House Dust Mite Allergen Extracts in Korea.Yonsei medical journal · 2021Article
- Cytotoxic effects of dental prosthesis grinding dust on RAW264.7 cells.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The abundance of lipopolysaccharide (LPS) in house dust mite (HDM) preparations is broad and mirrors the variability seen in the homes of people with asthma. LPS in commercially available stocks ranges from 31 to 5,2000 endotoxin units. The influence of vastly different LPS loads on the mechanisms that define the immune and inflammatory phenotype of HDM-challenged mice has not been defined. This aim of the study was to understand the lung phenotype of mice challenged with HDM extract containing high or low levels of LPS. Female BALB/c mice were sensitized for 2 wk with commercial HDM extract containing either high (36,000 endotoxin units; HHDM) or low (615 endotoxin units; LHDM) levels of LPS. Lung phenotype was characterized by measuring lung function, total and differential cell counts, cytokine abundance, and the lung transcriptome by RNA-sequencing. LPS levels in HDM stocks used for preclinical asthma research in mice remain poorly reported. In 2019, only 14% of papers specified LPS concentration in HDM lots. Specific differences existed in airway responsiveness between mice challenged with HHDM or LHDM. HHDM- and LHDM-induced cytokine profiles of bronchial lavage were significantly different and the lung transcriptome was differentially enriched for genes involved in DNA damage repair or cilium movement, following HHDM or LHDM challenge, respectively. The abundance of LPS in commercially available HDM influences the phenotype of allergic airways inflammation in mice. Failure to report the level of LPS in HDM extracts used in animal models of airway disease will lead to inconsistency in reproducibility and reliability of published data.
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