ArticleInternational journal of molecular medicine2020
LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR‑346.
Article in International journal of molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- miR-524-5p suppresses cervical cancer progression through targeting the LRP6/Wnt/β-catenin axis.Journal of molecular histology · 2026Article
- The regulation of LRPs by miRNAs in cancer: influencing cancer characteristics and responses to treatment.Cancer cell international · 2025Review
- MiR-424-5p Inhibits Proliferation, Migration, Invasion and Angiogenesis of the HTR-8/SVneo Cells Through Targeting LRP6 Mediated β-catenin.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modifications.PloS one · 2024Article
- EZH2 enhances proliferation and migration of trophoblast cell lines by blocking GADD45A-mediated p38/MAPK signaling pathway.Bioengineered · 2022Article
- Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia.Frontiers in cell and developmental biology · 2022Review
- A 5`-tRNA Derived Fragment NamedtiRNA-Val-CAC-001 Works as a Suppressor in Gastric Cancer.Cancer management and research · 2022Article
- MicroRNA-513c-5p is involved in the pathogenesis of preeclampsia by regulating of low-density lipoprotein receptor-associated protein 6.BMC pregnancy and childbirth · 2021Article
- Identification of downstream targets and signaling pathways of long non-coding RNA NR_002794 in human trophoblast cells.Bioengineered · 2021Article
- Ubiquitin-Specific Peptidase 5 is Involved in the Proliferation of Trophoblast Cells by Regulating Wnt/β-Catenin Signaling.Molecular biotechnology · 2021Article
- Recent Advances of MicroRNAs, Long Non-coding RNAs, and Circular RNAs in Preeclampsia.Frontiers in physiology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low‑density lipoprotein receptor‑related protein 6 (LRP6) promotes metastasis in numerous types of cancer; however, its role in trophoblast cells has been less frequently reported. In the present study, the effects of up‑ and downregulation of LRP6 on trophoblast cells were investigated accordingly. The study aimed to develop a therapeutic target for gestational choriocarcinoma. The expression levels of LRP6 in pre‑eclampsia (PE) tissues, trophoblast cell lines and gestational choriocarcinoma cells were determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) assay. Double‑luciferase reporter analysis was conducted to detect the regulatory gene of LRP6. Furthermore, the proliferative, migratory and invasive abilities of trophoblasts and gestational choriocarcinoma cells were determined by CCK‑8, wound healing, and Transwell assays, respectively. The expression levels of the genes and proteins of interest [matrix metalloproteinase (MMP)‑2, MMP‑9, tissue inhibitor of metalloproteinase‑1 (TIMP‑1), and TIMP‑2] associated with tumor cell invasion were measured by performing RT‑qPCR and western blotting, respectively. The National Center for Biotechnology Information database revealed that LRP6 was relatively highly expressed in placental tissues, but was poorly expressed in PE tissues and trophoblast cell lines. The upregulation of LRP6 not only increased the activity, migration and invasion of trophoblast cells, but it also promoted the expression of MMP‑2 and MMP‑9, whereas it inhibited the expression levels of TIMP‑1 and TIMP‑2. Such results followed the opposite trend to those of downregulation of LRP6 in gestational choriocarcinoma cells. Moreover, LRP6 was predicted to be the target gene for microRNA (miR)‑346, which was highly expressed in PE tissues and trophoblast cell lines. The present study also revealed that LRP6 could reverse the effects of miR‑346 on the proliferation, migration and invasion of trophoblast cells. Therefore, considered collectively, the results of the present study have demonstrated that LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR‑346, and that LRP6 may serve as a potential target in cancer treatment.
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