ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2020
Identification of hypervirulent Klebsiella pneumoniae isolates using the string test in combination with Galleria mellonella infectivity.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 58 citations in OpenAlex.
- DisseminatedDEN open · 2027Article
- Not so innocent: Environmental Klebsiella pneumoniae disseminating resistance and virulence via conjugation.World journal of microbiology & biotechnology · 2026Article
- Genomic Analysis of HypervirulentIranian journal of biotechnology · 2026Article
- Comparative profiling of virulence determinants, capsular serotypes, and antimicrobial resistance in hypervirulent and classical Klebsiella pneumoniae isolates from liver abscess patients.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Genotypes, antibiotic resistance, and virulence diversity among Klebsiella pneumoniae species complex isolates in Taiwan.Gut pathogens · 2026Article
- Emergence of Carbapenem-Resistant Hypervirulent ST111 K63Polish journal of microbiology · 2026Article
- Emergence of a carbapenem-resistantFrontiers in cellular and infection microbiology · 2026Article
- Prevalence and Molecular Characterization of Carbapenemase and Hypervirulence Genes inInternational journal of microbiology · 2026Article
- Pathogenic genes associated with immune-related genes in graves' disease: a multi-omics Mendelian randomization analysis.Scientific reports · 2025Article
- Genomic Characterization of a Rare K30-ST198 HypervirulentInternational journal of molecular sciences · 2025Article
- Genomic Characterization of Multidrug-Resistant and ESBL-ProducingMicroorganisms · 2025Article
- Assessment of Hypervirulent Klebsiella pneumoniae Isolates Using Multilocus Sequence Typing in West of Iran.MicrobiologyOpen · 2025Article
- Phenotypic and Genotypic Characteristics of Hypervirulent Klebsiella pneumoniae (hvKp): A Narrative Review.Current microbiology · 2025Review
- Prevalence and molecular characteristics of colistin-resistant isolates among carbapenem-resistant Klebsiella pneumoniae in Central South China: a multicenter study.Annals of clinical microbiology and antimicrobials · 2025Article
- Emergence and clinical challenges of ST11-K64 carbapenem-resistant Klebsiella pneumoniae: molecular insights and implications for antimicrobial resistance and virulence in Southwest China.BMC infectious diseases · 2025Article
- Modulation of virulence and metabolic profiles inFrontiers in cellular and infection microbiology · 2025Article
- Singleplex real-time PCR and duplex PCR platform for the rapid detection of hypervirulentFrontiers in veterinary science · 2025Article
- Polymyxin-resistant hypervirulentFrontiers in microbiology · 2025Article
- Evaluation ofMicrobial genomics · 2024Article
- Pulmonary abscess secondary to epididymitis caused by extended spectrum β-lactamase-producing hypervirulent Klebsiella pneumoniae: a case report.BMC infectious diseases · 2024Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Distinguishing between hypervirulent Klebsiella pneumoniae (hvKp) and classical Klebsiella pneumoniae (cKp) is a challenge to clinical laboratories. The aim of this study was to determine the practicability of combining the G. mellonella killing assay with a string test to differentiate hvKp from cKp. One hundred and three clinical K. pneumoniae isolates were collected. PCR amplification and wzi sequencing were used to determine the capsular serotype. Virulence genes allS, iro, iuc, and rmpA2, used frequently to identify hvKp, were detected by PCR. The virulence of K. pneumoniae isolates was evaluated using the following assays in parallel: molecular markers detection, G. mellonella killing assay alone, G. mellonella killing assay combined with the string test, and mouse infection. The results showed that the sensitivity, specificity, positive predictive value, and negative predictive value of combining the G. mellonella killing assay with a string test were 95.56%, 94.83%, 93.48%, and 96.49%, respectively, compared with mouse infection used as a positive reference. These values were significantly greater than those obtained using the G. mellonella killing assay only. The sensitivity, specificity, positive predictive value, and negative predictive value of allS, iro, iuc, and rmpA2 were greater than 77.78%, but less than combining the G. mellonella killing assay and string test. G. mellonella killing assay used in conjugation with the string test is a relatively simple and accurate method to assess K. pneumoniae virulence and differentiate between hvKp and cKp.
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