ArticleBiology of reproduction2020
Fatty acid induced lipolysis influences embryo development, gene expression and lipid droplet formation in the porcine cumulus cells†.
Article in Biology of reproduction, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Metabolic control of feto-placental development and pregnancy outcomes.Nature reviews. Endocrinology · 2026Review
- Genome-Wide Association Studies on the Autosomes and Chromosome X Uncover Genetic Basis of Reproductive Traits in Yorkshire Pigs.Animals : an open access journal from MDPI · 2026Article
- Review
- Uterine histotroph and conceptus development. IV. Metabolomic analyses of uterine luminal fluid reveals regulatory landscapes during the peri-implantation period of pregnancy in pigs†.Biology of reproduction · 2025Article
- Micro magnetic resonance spectroscopy for noninvasive metabolic screening of mammalian embryos and oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Integrated Multi-Omics Analysis Reveals Key Regulators of Bovine Oocyte Maturation.International journal of molecular sciences · 2025Article
- Exposure ofFrontiers in veterinary science · 2025Article
- Impact of obesity on follicular fluid lipid composition and IVF/ICSI outcomes in Korean women: A lipidomic study.PloS one · 2025Article
- Metabolic and endocrinologic interplay in the peri-ovulatory follicle to support the cumulus-oocyte-complex towards full competence.Animal reproduction · 2025Review
- Identifying Significant SNPs of the Total Number of Piglets Born and Their Relationship with Leg Bumps in Pigs.Biology · 2024Article
- Ovarian aging: energy metabolism of oocytes.Journal of ovarian research · 2024Review
- Lithium Chloride Promotes Endogenous Synthesis of CLA in Bovine Mammary Epithelial Cells.Biological trace element research · 2024Article
- Mapping the lipidomic secretome of the early equine embryo.Frontiers in veterinary science · 2024Article
- Article
- Role of functional fatty acids in modulation of reproductive potential in livestock.Journal of animal science and biotechnology · 2023Review
- Species and embryo genome origin affect lipid droplets in preimplantation embryos.Frontiers in cell and developmental biology · 2023Article
- Article
- High-Fat Diet and Female Fertility across Lifespan: A Comparative Lesson from Mammal Models.Nutrients · 2022Review
- Glutathione deficiency decreases lipid droplet stores and increases reactive oxygen species in mouse oocytes†.Biology of reproduction · 2022Article
- Metabolism of fatty acids in follicular cells, oocytes, and blastocysts.Reproduction & fertility · 2022Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pig oocyte maturation protocol differs from other mammalian species due to dependence on follicular fluid (FF) supplementation. One of the most abundant components of the porcine follicular fluid are fatty acids (FAs). Although evidence from other mammalian models revealed a negative impact of saturated fatty acids (SFA) on developmental competence of oocytes, pig has not yet been widely analyzed. Therefore, we aimed to investigate whether supplementation of IVM medium with 150 μM of stearic acid (SA) and oleic acid (OA) affects lipid content and expression of genes related to fatty acid metabolism in porcine cumulus-oocyte complexes and parthenogenetic embryo development. We found significant influence of fatty acids on lipid metabolism in cumulus cells without affecting the oocyte proper. The expression of ACACA, SCD, PLIN2, FADS1, and FADS2 genes was upregulated (P < 0.01) in cumulus cells, while their expression in oocytes did not change. The increase in gene expression was more pronounced in the case of OA (e.g., up to 30-fold increase in PLIN2 transcript level compared to the control). The number of lipid droplets and occupied area increased significantly in the cumulus cells and did not change in oocytes after SA treatment. Oleic acid improved the blastocyst rate (48 vs 32% in control), whereas stearic acid did not affect this parameter (27%). Additionally, we have discovered a phenotypic diversity of LD in cumulus cells in response to FA supplementation, suggesting extensive lipolysis in response to SA. Stearic acid excess in maturation media led to the formation of multiple micro lipid droplets in cumulus cells.
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Registered trials
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