ArticleFrontiers in bioengineering and biotechnology2020
Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues.
Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Biomimetic villi-crypt scaffold-on-chip with tunable mechanical properties for intestinal epithelium modeling.Materials today. Bio · 2026Article
- Application and prospects of organoid-on-a-chip in research on the intestinal mucosal barrier.Burns & trauma · 2026Review
- Histological Processing of Scaffolds: Challenges and Solutions.Journal of functional biomaterials · 2025Review
- Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications.Small science · 2025Review
- Set Up and Utilization of a Three-Dimensional In Vitro Bioreactor System for Human Intestinal Studies and Microbial Co-Cultures.Current protocols · 2024Article
- Printing channels with millimeter-scale curvature and deciphering their effect on the proliferation, morphology, orientation, and migration of M-22 cells.International journal of bioprinting · 2023Article
- Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium.ACS biomaterials science & engineering · 2022Article
- Bioengineered 3D Tissue Model of Intestine Epithelium with Oxygen Gradients to Sustain Human Gut Microbiome.Advanced healthcare materials · 2022Article
- Mimicking the Intestinal Host-Pathogen Interactions in a 3D In Vitro Model: The Role of the Mucus Layer.Pharmaceutics · 2022Article
- Recent Research on Hybrid Hydrogels for Infection Treatment and Bone Repair.Gels (Basel, Switzerland) · 2022Review
- Appreciating the role of cell shape changes in the mechanobiology of epithelial tissues.Biophysics reviews · 2022Review
- Processing of fast-gelling hydrogel precursors in microfluidics by electrocoalescence of reactive species.Soft matter · 2021Article
- Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D.Advanced healthcare materials · 2021Article
- Manufacturing micropatterned collagen scaffolds with chemical-crosslinking for development of biomimetic tissue-engineered oral mucosa.Scientific reports · 2020Article
- In vitro models of intestinal epithelium: Toward bioengineered systems.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While conventional cell culture methodologies have relied on flat, two-dimensional cell monolayers, three-dimensional engineered tissues are becoming increasingly popular. Often, engineered tissues can mimic the complex architecture of native tissues, leading to advancements in reproducing physiological functional properties. In particular, engineered intestinal tissues often use hydrogels to mimic villi structures. These finger-like protrusions of a few hundred microns in height have a well-defined topography and curvature. Here, we examined the cell morphological response to these villus-like microstructures at single-cell resolution using a novel embedding method that allows for the histological processing of these delicate hydrogel structures. We demonstrated that by using photopolymerisable poly(ethylene) glycol as an embedding medium, the villus-like microstructures were successfully preserved after sectioning with vibratome or cryotome. Moreover, high-resolution imaging of these sections revealed that cell morphology, nuclei orientation, and the expression of epithelial polarization markers were spatially encoded along the vertical axis of the villus-like microstructures and that this cell morphological response was dramatically affected by the substrate curvature. These findings, which are in good agreement with the data reported for
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.