ArticleScientific reports2020
Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Multiscale Architecture and Mechanics of the Cell Nucleus: Implications for Disease, Bioengineering and Nanomedicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Stochastic motility energetics reveals cooperative bacterial swarming in optical tweezers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Viscoelastic differences between isolated and live MCF7 cancer cell nuclei resolved with AFM microrheology.Journal of the Royal Society, Interface · 2025Article
- Comparative Study of Cell Nuclei Segmentation Based on Computational and Handcrafted Features Using Machine Learning Algorithms.Diagnostics (Basel, Switzerland) · 2025Article
- Red blood cell flickering activity locally controlled by holographic optical tweezers.iScience · 2024Article
- Microscopy methods to visualize nuclear organization in biomechanical studies.Current opinion in biomedical engineering · 2024Article
- Article
- Article
- When nasal cytology detects acute lymphoblastic leukaemia: New diagnostical implications.Cytopathology : official journal of the British Society for Clinical Cytology · 2022Article
- MPTHub: An Open-Source Software for Characterizing the Transport of Particles in Biorelevant Media.Nanomaterials (Basel, Switzerland) · 2022Article
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- Tumor cell nuclei soften during transendothelial migration.Journal of biomechanics · 2021Article
- The Cancer Microenvironment: Mechanical Challenges of the Metastatic Cascade.Frontiers in bioengineering and biotechnology · 2021Review
- Review
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The nucleus is fundamentally composed by lamina and nuclear membranes that enclose the chromatin, nucleoskeletal components and suspending nucleoplasm. The functional connections of this network integrate external stimuli into cell signals, including physical forces to mechanical responses of the nucleus. Canonically, the morphological characteristics of the nucleus, as shape and size, have served for pathologists to stratify and diagnose cancer patients; however, novel biophysical techniques must exploit physical parameters to improve cancer diagnosis. By using multiple particle tracking (MPT) technique on chromatin granules, we designed a SURF (Speeded Up Robust Features)-based algorithm to study the mechanical properties of isolated nuclei and in living cells. We have determined the apparent shear stiffness, viscosity and optical density of the nucleus, and how the chromatin structure influences on these biophysical values. Moreover, we used our MPT-SURF analysis to study the apparent mechanical properties of isolated nuclei from patients of acute lymphoblastic leukemia. We found that leukemia cells exhibited mechanical differences compared to normal lymphocytes. Interestingly, isolated nuclei from high-risk leukemia cells showed increased viscosity than their counterparts from normal lymphocytes, whilst nuclei from relapsed-patient's cells presented higher density than those from normal lymphocytes or standard- and high-risk leukemia cells. Taken together, here we presented how MPT-SURF analysis of nuclear chromatin granules defines nuclear mechanical phenotypic features, which might be clinically relevant.
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