ArticleScientific reports2020
Single-molecule long-read sequencing of the full-length transcriptome of Rhododendron lapponicum L.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 47 citations in OpenAlex.
- Selection and Validation of Stable Reference Genes for Accurate qRT-PCR Analysis of Flower Color Development inCurrent issues in molecular biology · 2026Article
- Full-length transcriptome sequencing analysis and characterization of MADS-box genes in Rhododendron fortunei.BMC plant biology · 2026Article
- Deciphering the Biosynthetic Pathways and Regulatory Networks of the Active Components ofInternational journal of molecular sciences · 2026Article
- Deciphering the genetic basis of chlorophyll fluorescence parameters and vegetation indices under heat stress in blueberry.BMC plant biology · 2025Article
- SSR Marker Acquisition and Application from Transcriptome of Captive Chinese Forest Musk Deer (Moschus berezovskii).Biochemical genetics · 2024Article
- Review
- Characterization and analysis of multi-organ full-length transcriptomes in Sphaeropteris brunoniana and Alsophila latebrosa highlight secondary metabolism and chloroplast RNA editing pattern of tree ferns.BMC plant biology · 2024Article
- Full-length transcriptome characterization ofFrontiers in genetics · 2024Article
- Full-Length Transcriptome Sequencing Analysis and Characterization of WRKY Transcription Factors Responsive to Cadmium Stress inPlants (Basel, Switzerland) · 2023Article
- Full-Length Transcriptome Sequencing Reveals the Molecular Mechanism ofAntioxidants (Basel, Switzerland) · 2023Article
- Integrative Analysis of Metabolome and Transcriptome Reveals Molecular Insight into Metabolomic Variations during Hawthorn Fruit Development.Metabolites · 2023Article
- Screening and identification of multiple abiotic stress responsive candidate genes based on hybrid-sequencing inHeliyon · 2023Article
- Full-length transcriptome analysis ofPeerJ · 2023Article
- Microsatellite analysis and polymorphic marker development based on the full-length transcriptome of Camellia chekiangoleosa.Scientific reports · 2022Article
- Single-molecule real-time sequencing of the full-length transcriptome of Halophila beccarii.Scientific reports · 2022Article
- Identification and genetic diversity analysis of hybrid offspring of azalea based on EST-SSR markers.Scientific reports · 2022Article
- Full-length transcriptome analysis ofPeerJ · 2022Article
- A PacBio single molecule real-time sequencing-based full-length transcriptome atlas of tree tomato (Frontiers in plant science · 2022Article
- Article
- The Rhododendron Plant Genome Database (RPGD): a comprehensive online omics database for Rhododendron.BMC genomics · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhododendron lapponicum L. is a familiar ornamental plant worldwide with important ornamental and economic value. However, a full-length R. lapponicum transcriptome is still lacking. In the present study, we used the Pacific Biosciences single-molecule real-time sequencing technology to generate the R. lapponicum transcriptome. A total of 346,270 full-length non-chimeric reads were generated, from which we obtained 75,002 high-quality full-length transcripts. We identified 55,255 complete open reading frames, 7,140 alternative splicing events and 2,011 long non-coding RNAs. In gene annotation analyses, 71,155, 33,653, 30,359 and 31,749 transcripts were assigned to the Nr, GO, COG and KEGG databases, respectively. Additionally, 3,150 transcription factors were detected. KEGG pathway analysis showed that 96 transcripts were identified coding for the enzymes associated with anthocyanin synthesis. Furthermore, we identified 64,327 simple sequence repeats from 45,319 sequences, and 150 pairs of primers were randomly selected to develop SSR markers. This study provides a large number of full-length transcripts, which will facilitate the further study of the genetics of R. lapponicum.
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