ArticleMicrobial cell factories2020
Synthesis of the character impact compound raspberry ketone and additional flavoring phenylbutanoids of biotechnological interest with Corynebacterium glutamicum.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Optimizing MatB-MatC-Dependent Malonyl-CoA Supply for Enhanced Raspberry Ketone Production inBiotech (Basel (Switzerland)) · 2026Article
- A high-sensitivity strategy to screen NAD(P)H-dependent reductase activity by coupled enzyme cascade.The Journal of biological chemistry · 2026Article
- Engineering of Corynebacterium glutamicum for the synthesis of aromatic compounds.Applied microbiology and biotechnology · 2025Review
- A review on natural phenylbutanoid attractants: Occurrence, distribution, and role in nature, especially in relation to Dacini fruit fly behavior and pollination.Journal of chemical ecology · 2024Review
- A type III polyketide synthase cluster in the phylum Planctomycetota is involved in alkylresorcinol biosynthesis.Applied microbiology and biotechnology · 2024Article
- Raspberry Ketone Accumulation inJournal of agricultural and food chemistry · 2023Article
- Sustainable biosynthetic pathways to value-added bioproducts from hydroxycinnamic acids.Applied microbiology and biotechnology · 2023Review
- Modular Metabolic Engineering and Synthetic Coculture Strategies for the Production of Aromatic Compounds in Yeast.ACS synthetic biology · 2023Article
- A whiff of the future: functions of phenylalanine-derived aroma compounds and advances in their industrial production.Plant biotechnology journal · 2022Review
- Application of Corynebacterium glutamicum engineering display system in three generations of biorefinery.Microbial cell factories · 2022Review
- Glucose-Derived Raspberry Ketone Produced via EngineeredFrontiers in bioengineering and biotechnology · 2022Article
- Engineered biosynthesis of plant polyketides by type III polyketide synthases in microorganisms.Frontiers in bioengineering and biotechnology · 2022Review
- Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants.Metabolic engineering communications · 2021Article
- Pharmacological Exploration of Phenolic Compound: Raspberry Ketone-Update 2020.Plants (Basel, Switzerland) · 2021Review
- Metabolic engineering of Escherichia coli for de novo production of 3-phenylpropanol via retrobiosynthesis approach.Microbial cell factories · 2021Article
- Sustainable Production ofMicroorganisms · 2021Article
- Physiological Response ofMicroorganisms · 2020Article
- Engineering intracellular malonyl-CoA availability in microbial hosts and its impact on polyketide and fatty acid synthesis.Applied microbiology and biotechnology · 2020Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe phenylbutanoid 4-(4-hydroxyphenyl)butan-2-one, commonly known as raspberry ketone, is responsible for the typical scent and flavor of ripe raspberries. Chemical production of nature-identical raspberry ketone is well established as this compound is frequently used to flavor food, beverages and perfumes. However, high demand for natural raspberry ketone, but low natural abundance in raspberries, render raspberry ketone one of the most expensive natural flavoring components.
resultsIn this study, Corynebacterium glutamicum was engineered for the microbial synthesis of the character impact compound raspberry ketone from supplemented p-coumaric acid. In this context, the NADPH-dependent curcumin/dihydrocurcumin reductase CurA from Escherichia coli was employed to catalyze the final step of raspberry ketone synthesis as it provides a hitherto unknown benzalacetone reductase activity. In combination with a 4-coumarate: CoA ligase from parsley (Petroselinum crispum) and a monofunctional benzalacetone synthase from Chinese rhubarb (Rheum palmatum), CurA constitutes the synthetic pathway for raspberry ketone synthesis in C. glutamicum. The resulting strain accumulated up to 99.8 mg/L (0.61 mM) raspberry ketone. In addition, supplementation of other phenylpropanoids allowed for the synthesis of two other naturally-occurring and flavoring phenylbutanoids, zingerone (70 mg/L, 0.36 mM) and benzylacetone (10.5 mg/L, 0.07 mM).
conclusionThe aromatic product portfolio of C. glutamicum was extended towards the synthesis of the flavoring phenylbutanoids raspberry ketone, zingerone and benzylacetone. Key to success was the identification of CurA from E. coli having a benzalacetone reductase activity. We believe, that the constructed C. glutamicum strain represents a versatile platform for the production of natural flavoring phenylbutanoids at larger scale.
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