Evidence map›Paper›PMID 32316600›Full record

ArticleInternational journal of molecular sciences2020

Salivary Small Extracellular Vesicles Associated miRNAs in Periodontal Status-A Pilot Study.

Pingping Han, Peter Mark Bartold, Carlos Salomon, Saso Ivanovski

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 77 citations in OpenAlex.

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  19. Emerging roles of exosomes in oral diseases progression.International journal of oral science · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 3 countries.

Pingping HanSchool of Dentistry, The University of Queensland, Brisbane, QLD 4006, Australia.ORCID 0000-0001-7546-1635
Peter Mark BartoldSchool of Dentistry, The University of Adelaide, Adelaide, SA 5000, Australia.ORCID 0000-0002-5695-3877
Carlos SalomonExosome Biology Laboratory, Centre for Clinical Diagnostics, the University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital, The University of Queensland, Brisbane, QLD 4029, Australia.ORCID 0000-0003-4474-0046
Saso IvanovskiSchool of Dentistry, The University of Queensland, Brisbane, QLD 4006, Australia.ORCID 0000-0001-5339-0936
The University of Queensland · AUThe University of Adelaide · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This pilot study aims to investigate whether salivary small extracellular vesicle (sEV)-associated microRNAs could act as potential biomarkers for periodontal disease status. Twenty-nine participants (10 who were healthy, nine with gingivitis, 10 with stage III/IV periodontitis) were recruited and unstimulated whole saliva samples were collected. Salivary sEVs were isolated using the size-exclusion chromatography (SEC) method and characterised by morphology, EV-protein and size distribution using transmission electron microscopy (TEM), Western Blot and Nanoparticle Tracking Analysis (NTA), respectively. Ten mature microRNAs (miRNAs) in salivary sEVs and saliva were evaluated using RT-qPCR. The discriminatory power of miRNAs as biomarkers in gingivitis and periodontitis versus healthy controls was evaluated by Receiver Operating Characteristics (ROC) curves. Salivary sEVs were comparable to sEVs morphology, mode, size distribution and particle concentration in healthy, gingivitis and periodontitis patients. Compared to miRNAs in whole saliva, three significantly increased miRNAs (hsa-miR-140-5p, hsa-miR-146a-5p and hsa-miR-628-5p) were only detected in sEVs in periodontitis when compared to that of healthy controls, with a good discriminatory power (area under the curve (AUC) = 0.96) for periodontitis diagnosis. Our study demonstrated that salivary sEVs are a non-invasive source of miRNAs for periodontitis diagnosis. Three miRNAs that are selectively enriched in sEVs, but not whole saliva, could be potential biomarkers for periodontal disease status.

Indexed as

AdultAgedChromatography, GelExtracellular VesiclesFemaleGenetic MarkersGingivitisHumansMaleMicroRNAsMiddle AgedPeriodontitisPilot ProjectsSalivaYoung AdultGenetic MarkersMicroRNAsMirn140 microRNA, humanMIRN146 microRNA, humanMIRN628 microRNA, humanbiomarker discoverymiRNAsperiodontal diseasesalivary small extracellular vesiclessize exclusion chromatography

Identifiers

PMID32316600
PMCPMC7215885
OpenAlexW3016415602

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.