ArticleJournal of neuroscience methods2020
Isolation, culture, and downstream characterization of primary microglia and astrocytes from adult rodent brain and spinal cord.
Article in Journal of neuroscience methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- A Primary Spinal Cord Mixed Culture Method for In Vitro Analysis of Glial Heterogeneity and Inflammatory Responses.Neurochemical research · 2026Article
- Lipocalin-2 deficiency attenuates kainic acid-induced hippocampal cell death in a high-fat diet-fed diabetic mice.Metabolic brain disease · 2026Article
- Method for the simultaneous isolation of primary astrocytes and microglia from the neonatal rats cerebral cortex.Frontiers in cellular neuroscience · 2026Article
- Ptbp1 knockdown induces conversion of rat spinal cord astrocytes into neuron like cells.Scientific reports · 2025Article
- Astrocytic spermidine insufficiency contributes to enhanced pain sensitivity associated with ApoE4.The journal of headache and pain · 2025Article
- In vitro models of microglia: a comparative study.Scientific reports · 2025Article
- The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1.Journal of virology · 2025Article
- CNS resident macrophages exhibit region-specific states and immunogenic responses during Rbpj-deficient brain arteriovenous malformation.Scientific reports · 2025Article
- Astrocytic Acid-Sensing Ion Channel 1a Contributes to the Development of Epileptic Cognitive Impairment.Biomolecules · 2025Article
- Isolation of Primary Brain Cells: Challenges and Solutions.Archives of clinical and biomedical research · 2025Article
- Pain research in a petri dish? Advantages and limitations of neuro-glial primary cell cultures from structures of the nociceptive system.Brain, behavior, & immunity - health · 2024Article
- Desloratadine ameliorates paclitaxel-induced peripheral neuropathy and hypersensitivity reactions in mice.Acta pharmacologica Sinica · 2024Article
- Mouse serum albumin induces neuronal apoptosis and tauopathies.Acta neuropathologica communications · 2024Article
- Current methods for the microglia isolation: Overview and comparative analysis of approaches.Cell and tissue research · 2024Review
- Preparation of Healthy Single Neuron or Astrocyte Suspension from Adult Mouse Brain for RNA-seq.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Established and emerging techniques for the study of microglia: visualization, depletion, and fate mapping.Frontiers in cellular neuroscience · 2024Review
- Translational models of stress and resilience: An applied neuroscience methodology review.Neuroscience applied · 2024Review
- Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis.Cell reports · 2023Article
- Assembling Spheroids of Rat Primary Neurons Using a Stress-Free 3D Culture System.International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundNeuroimmunologists aspire to understand the interactions between neurons, microglia, and astrocytes in the CNS. To study these cells, researchers work with either immortalized cell lines or primary cells acquired from animal tissue. Primary cells reflect in vivo characteristics and functionality compared to immortalized cells; however, they are challenging to acquire and maintain. NEW
methodEstablished protocols to harvest primary glia use neonatal rodents, here we provide a method for simultaneously isolating microglia and astrocytes from brain and/or spinal cord from adult rodents. We utilized a discontinuous percoll density gradient enabling easy discrimination of these cell populations without enzymatic digestion or complex sorting techniques.
resultsWe found cells isolated from the percoll interface between 70 %-50 % were microglia, as they express ionizing calcium-binding adaptor molecule 1 (Iba1) in immunocytochemistry and CD11b COMPARISON WITH EXISTING
methodsOur method allows for rapid isolation of both microglia and astrocytes in one protocol with relatively few resources, preserves cellular phenotype, and yields high cell numbers without magnetic or antibody sorting.
conclusionHere we show a novel, single protocol to isolate microglia and astrocytes from brain and spinal cord tissue, allowing for culturing and other downstream applications from the cells of animals of various ages, which will be useful for researchers investigating these two major glial cell types from the brain or spinal cord of the same rodent.
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Registered trials
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