ArticlePloS one2020
Persistent contamination of raw milk by Campylobacter jejuni ST-883.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Novel AlphaPlex Design Enables Rapid Differentiation ofPathogens (Basel, Switzerland) · 2026Article
- A comprehensive review of foodborne disease burden associated with protein foods: a 13-year evaluation of incidence and outbreaks in high-income countries.Italian journal of food safety · 2026Article
- Genomic insights and survival dynamics ofFrontiers in microbiology · 2026Article
- Raw Milk as a Source ofPathogens (Basel, Switzerland) · 2025Article
- Transmission pathways of Campylobacter jejuni between humans and livestock in rural Ethiopia are highly complex and interdependent.Gut pathogens · 2025Article
- How Long Do Microorganisms Survive and Persist in Food? A Systematic Review.Microorganisms · 2025Review
- Aerotolerancy ofPathogens (Basel, Switzerland) · 2024Review
- Campylobacteriosis: A rising threat in foodborne illnesses.Open veterinary journal · 2024Review
- The Transmission ofPathogens (Basel, Switzerland) · 2024Article
- Persistence of microbiological hazards in food and feed production and processing environments.EFSA journal. European Food Safety Authority · 2024Article
- Genomic diversity of Campylobacter jejuni and Campylobacter coli isolated from the Ethiopian dairy supply chain.PloS one · 2024Article
- Metabolic Pathways for the Biosynthesis of Heptoses Used in the Construction of Capsular Polysaccharides in the Human PathogenBiochemistry · 2023Article
- Review
- Circular intermediate-mediated horizontal transfer of the chromosome-encodedFrontiers in microbiology · 2023Article
- Growth kinetics and fitness of fluoroquinolone resistant and susceptibleFrontiers in veterinary science · 2023Article
- Suppression of pathogens in properly refrigerated raw milk.PloS one · 2023Article
- Functional Characterization of a HAD Phosphatase Involved in Capsular Polysaccharide Biosynthesis inBiochemistry · 2022Article
- Discovery and Functional Characterization of a Clandestine ATP-Dependent Amidoligase in the Biosynthesis of the Capsular Polysaccharide fromBiochemistry · 2022Article
- The Use of Ozone as an Eco-Friendly Strategy against Microbial Biofilm in Dairy Manufacturing Plants: A Review.Microorganisms · 2022Review
- High Prevalence of Intestinal Pathogens in Indigenous in Colombia.Journal of clinical medicine · 2020Article
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Authors and funding
5 authors.
Funding
Abstract
Campylobacter jejuni has caused several campylobacteriosis outbreaks via raw milk consumption. This study reports follow-up of a milk-borne campylobacteriosis outbreak that revealed persistent C. jejuni contamination of bulk tank milk for seven months or longer. Only the outbreak-causing strain, representing sequence type (ST) 883, was isolated from milk, although other C. jejuni STs were also isolated from the farm. We hypothesized that the outbreak strain harbors features that aid its environmental transmission or survival in milk. To identify such phenotypic features, the outbreak strain was characterized for survival in refrigerated raw milk and in aerobic broth culture by plate counting and for biofilm formation on microplates by crystal violet staining and quantification. Furthermore, whole-genome sequences were studied for such genotypic features. For comparison, we characterized isolates representing other STs from the same farm and an ST-883 isolate that persisted on another dairy farm, but was not isolated from bulk tank milk. With high inocula (105 CFU/ml), ST-883 strains survived in refrigerated raw milk longer (4-6 days) than the other strains (≤3 days), but the outbreak strain showed no outperformance among ST-883 strains. This suggests that ST-883 strains may share features that aid their survival in milk, but other mechanisms are required for persistence in milk. No correlation was observed between survival in refrigerated milk and aerotolerance. The outbreak strain formed a biofilm, offering a potential explanation for persistence in milk. Whether biofilm formation was affected by pTet-like genomic element and phase-variable genes encoding capsular methyltransferase and cytochrome C551 peroxidase warrants further study. This study suggests a phenotypic target candidate for interventions and genetic markers for the phenotype, which should be investigated further with the final aim of developing control strategies against C. jejuni infections.
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