ArticleBiochemistry2020
A Phosphorylated Intermediate in the Activation of WNK Kinases.
Article in Biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Structural Basis for Potassium Inhibition of WNK Kinases.Biochemistry · 2026Article
- Structural basis for pseudokinase-mediated regulation of GCN2 in the integrated stress response.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Navigating the multifaceted intricacies of the NaPhysiological reviews · 2024Review
- Article
- WNK1 promotes water homeostasis by acting as a central osmolality sensor for arginine vasopressin release.The Journal of clinical investigation · 2023Article
- The Post-Translational Modification Networking in WNK-Centric Hypertension Regulation and Electrolyte Homeostasis.Biomedicines · 2022Review
- CCT and CCT-Like Modular Protein Interaction Domains in WNK Signaling.Molecular pharmacology · 2022Review
- Sequence and structural variations determining the recruitment of WNK kinases to the KLHL3 E3 ligase.The Biochemical journal · 2022Article
- Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.Nature communications · 2021Article
- Osmosensing by WNK Kinases.Molecular biology of the cell · 2021Article
- LRRC8A reduces intracellular chloride to permit WNK activation in response to hypertonic stress.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- WNKs are potassium-sensitive kinases.American journal of physiology. Cell physiology · 2021Article
- WNK3 and WNK4 exhibit opposite sensitivity with respect to cell volume and intracellular chloride concentration.American journal of physiology. Cell physiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
WNK kinases autoactivate by autophosphorylation. Crystallography of the kinase domain of WNK1 phosphorylated on the primary activating site (pWNK1) in the presence of AMP-PNP reveals a well-ordered but inactive configuration. This new pWNK1 structure features specific and unique interactions of the phosphoserine, less hydration, and smaller cavities compared with those of unphosphorylated WNK1 (uWNK1). Because WNKs are activated by osmotic stress in cells, we addressed whether the structure was influenced directly by osmotic pressure. pWNK1 crystals formed in PEG3350 were soaked in the osmolyte sucrose. Suc-WNK1 crystals maintained X-ray diffraction, but the lattice constants and pWNK1 structure changed. Differences were found in the activation loop and helix C, common switch loci in kinase activation. On the basis of these structural changes, we tested for effects on
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.