Evidence map›Paper›PMID 32314126›Full record

ArticleCellular and molecular neurobiology2021

Inhibition of Ferroptosis Alleviates Early Brain Injury After Subarachnoid Hemorrhage In Vitro and In Vivo via Reduction of Lipid Peroxidation.

Yuchen Li, Yao Liu, Pei Wu, Yang Tian, Binbing Liu, Jinju Wang, Ji Bihl, Huaizhang Shi

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular neurobiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 78 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
78citing papers in PubMed, 1 pooled it
11.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

78 citing papers in PubMed, 1 synthesis or guideline pooled it, 136 citations in OpenAlex.

  1. Pooled it
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  6. Impact of Oxidative Stress-Driven Ferroptosis in Neurodegeneration.International journal of molecular sciences · 2026
    Review
  7. Regulation of microglial activation by progranulin through the NF-κB pathway in subarachnoid hemorrhage and its effect on white matter injury.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2026
    Article
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18 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Yuchen LiDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150000, Heilongjiang, China.
Yao LiuDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150000, Heilongjiang, China.
Pei WuDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150000, Heilongjiang, China.
Yang TianDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150000, Heilongjiang, China.
Binbing LiuDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150000, Heilongjiang, China.
Jinju WangDepartment of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, OH, 45435, USA.
Ji Bihl *Department of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, OH, 45435, USA. ji.bihl@wright.edu.
Huaizhang Shi *Department of Neurosurgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150000, Heilongjiang, China. huaizhangshi@126.com.
First Affiliated Hospital of Harbin Medical University · CNWright State University · USHarbin Medical University · CN

Funding

American Heart Association 16SDG26420078Innovation Fund of Harbin Medical University YTSKYCX2018-38HYDNatural Science Foundation of Heilongjiang Province ZD2018018
6 · The paper itself

Abstract

Subarachnoid hemorrhage (SAH) is a serious cerebrovascular disease with high mortality, and the mean age at morbidity is younger than in other types of stroke. Early brain injury (EBI) plays a key role in the poor prognoses of SAH. In EBI, multiple forms of cell death have been identified and well studied; however, the role of ferroptosis has not been elucidated. Hence, in this study, we developed an in vivo (SAH rat model) and in vitro model (SH-SY5Y oxyhemoglobin injury model) to understand the role of ferroptosis in EBI, then explored the protective mechanism of ferrostatin-1 (Fer-1). Firstly, we found that neurological scores, blood-brain barrier permeability, brain edema deteriorated after SAH in the in vivo model, cell viability was decreased after SAH in both cortex and SH-SY5Y cells. Further, iron content in cortex was increased after SAH, while transferrin receptor 1 and ferroportin (Fpn) were increased in oxyhemoglobin-treated in vitro model. Additionally, glutathione content and glutathione peroxidase 4 activity were reduced in SAH rats, and lipid peroxides were increased in the oxyhemoglobin-treated cells. Finally, administration of Fer-1 upregulated Fpn and decreased the iron content, then improved the lipid peroxidation and EBI. However, Fer-1 had no effect on the apoptosis. Our study indicated that the ferroptosis was involved in EBI of SAH, and the inhibitor Fer-1 provided neuroprotection against EBI by alleviating ferroptosis, the potential protective mechanism might be via suppressing lipid peroxidation.

Indexed as

FerroptosisLipid PeroxidationAnimalsBlood-Brain BarrierBrain EdemaBrain InjuriesCell DeathCell Line, TumorCell SurvivalHumansIronMaleMitochondriaNeuronsPermeabilityRatsIronEarly brain injuryFerostatin-1FerroptosisLipid peroxidationSubarachnoid hemorrhage

Identifiers

PMID32314126
PMCPMC11448630
OpenAlexW3016651431

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.