ArticleAMB Express2020
Identification of genes involved in xylose metabolism of Meyerozyma guilliermondii and their genetic engineering for increased xylitol production.
Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Dimorphism of oleaginous yeast Meyerozyma guilliermondii during single-cell oil production under decanoic acid stress.World journal of microbiology & biotechnology · 2026Article
- Current trends in the production of xylitol and paving the way for metabolic engineering in microbes.Biotechnology for biofuels and bioproducts · 2025Review
- Genomic characteristics and genetic manipulation of the marine yeast Scheffersomyces spartinae.Applied microbiology and biotechnology · 2024Article
- Research progress in the biosynthesis of xylitol: feedstock evolution from xylose to glucose.Biotechnology letters · 2024Review
- Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery.Microbial cell factories · 2023Article
- Biological production of xylitol by using nonconventional microbial strains.World journal of microbiology & biotechnology · 2022Review
- Lignocellulosic xylitol production from corncob using engineeredFrontiers in bioengineering and biotechnology · 2022Article
- Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries.Sustainable energy & fuels · 2021Review
- Nectar Yeast Community of Tropical Flowering Plants and Assessment of Their Osmotolerance and Xylitol-Producing Potential.Current microbiology · 2021Article
- Global Transcriptome Profile of the Oleaginous YeastJournal of fungi (Basel, Switzerland) · 2021Article
- Selection of Potential Yeast Probiotics and a Cell Factory for Xylitol or Acid Production from Honeybee Samples.Metabolites · 2021Article
- Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism.Foods (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Meyerozyma guilliermondii, a non-conventional yeast that naturally assimilates xylose, is considered as a candidate for biotechnological production of the sugar alternative xylitol. Because the genes of the xylose metabolism were yet unknown, all efforts published so far to increase the xylitol yield of this yeast are limited to fermentation optimization. Hence, this study aimed to genetically engineer this organism for the first time with the objective to increase xylitol production. Therefore, the previously uncharacterized genes of M. guilliermondii ATCC 6260 encoding for xylose reductase (XR) and xylitol dehydrogenase (XDH) were identified by pathway investigations and sequence similarity analysis. Cloning and overexpression of the putative XR as well as knockout of the putative XDH genes generated strains with about threefold increased xylitol yield. Strains that combined both genetic modifications displayed fivefold increase in overall xylitol yield. Enzymatic activity assays with lysates of XR overexpressing and XDH knockout strains underlined the presumed functions of the respective genes. Furthermore, growth evaluation of the engineered strains on xylose as sole carbon source provides insights into xylose metabolism and its utilization for cell growth.
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Registered trials
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