Evidence map›Paper›PMID 32314041›Full record

ArticleCancer immunology, immunotherapy : CII2020

Epstein-Barr virus (EBV) and polyomaviruses are detectable in oropharyngeal cancer and EBV may have prognostic impact.

Timo Carpén, Stina Syrjänen, Lauri Jouhi, Reija Randen-Brady, Caj Haglund, Antti Mäkitie, Petri S Mattila, Jaana Hagström

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer immunology, immunotherapy : CII, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
1.7field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Immune biomarkers for head and neck cancer.Cancer immunology, immunotherapy : CII · 2025
    Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Could MMP3 and MMP9 Serve as Biomarkers in EBV-Related Oropharyngeal Cancer.International journal of molecular sciences · 2024
    Article
  10. Article
  11. Review
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. The Oral Microbiome and Cancer.Frontiers in immunology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Timo CarpénDepartment of Otorhinolaryngology - Head and Neck Surgery, University of Helsinki and HUS Helsinki University Hospital, P.O. Box 263, 00029 HUS, Helsinki, Finland. timo.carpen@fimnet.fi.ORCID http://orcid.org/0000-0003-0117-8559
Stina SyrjänenDepartment of Oral Pathology and Oral Radiology, University of Turku and Department of Pathology, Turku University Hospital, Lemminkäisenkatu 2, 20520, Turku, Finland.ORCID http://orcid.org/0000-0003-0738-5698
Lauri JouhiDepartment of Otorhinolaryngology - Head and Neck Surgery, University of Helsinki and HUS Helsinki University Hospital, P.O. Box 263, 00029 HUS, Helsinki, Finland.ORCID http://orcid.org/0000-0003-1354-8027
Reija Randen-BradyDepartment of Pathology, University of Helsinki and HUS Helsinki University Hospital, P.O. Box 21, 00014 HUS, Helsinki, Finland.
Caj HaglundDepartment of Surgery, University of Helsinki and HUS Helsinki University Hospital, P.O. Box 440, 00029 HUS, Helsinki, Finland.ORCID http://orcid.org/0000-0003-0456-4965
Antti MäkitieDepartment of Otorhinolaryngology - Head and Neck Surgery, University of Helsinki and HUS Helsinki University Hospital, P.O. Box 263, 00029 HUS, Helsinki, Finland.ORCID http://orcid.org/0000-0002-0451-2404
Petri S MattilaDepartment of Otorhinolaryngology - Head and Neck Surgery, University of Helsinki and HUS Helsinki University Hospital, P.O. Box 263, 00029 HUS, Helsinki, Finland.ORCID http://orcid.org/0000-0003-1911-1811
Jaana HagströmDepartment of Pathology, University of Helsinki and HUS Helsinki University Hospital, P.O. Box 21, 00014 HUS, Helsinki, Finland.ORCID http://orcid.org/0000-0001-6079-7881
University of Helsinki · FIKarolinska University Hospital · SEUniversity of Turku · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe etiological role of human papillomavirus (HPV) in oropharyngeal squamous cell carcinoma (OPSCC) is confirmed. However, the role of other oncoviruses in OPSCC is unknown. MATERIALS AND

methodsA total of 158 consecutive OPSCC patients treated with curative intent were included. DNA extracted from tumor sections was used to detect Epstein-Barr virus (EBV), HPV, and the following polyomaviruses: John Cunningham virus (JCV), Simian virus 40 (SV40), and BK virus (BKV) with PCR. In addition, p16 expression was studied by immunohistochemistry, and EBV-encoded small RNA (EBER) transcripts were localized by in situ hybridization. The effect of viral status on overall survival (OS) and disease-free survival (DFS) was analyzed.

resultsA total of 94/158 samples (59.5%) were HPV-positive, 29.1% contained BKV DNA, 20.3% EBV DNA, 13.9% JCV DNA, and 0.6% SV40 DNA. EBER was expressed only in stromal lymphocytes adjacent to the tumor and correlated with HPV positivity (p = 0.026). p16 expression associated only with HPV. None of the three polyomaviruses had an impact on survival. Patients with EBER-positive but HPV-negative OPSCC had significantly poorer OS and DFS than those with HPV-positive OPSCC and slightly worse prognosis compared with the patients with EBER-negative and HPV-negative OPSCC.

conclusionPolyomaviruses are detectable in OPSCC but seem to have no impact on survival, whereas HPV was the strongest viral prognostic factor. EBER expression, as a sign of latent EBV infection, may have prognostic impact among patients with HPV-negative OPSCC. EBER analysis may identify a new subgroup of OPSCCs unrelated to HPV.

Indexed as

Biomarkers, TumorCarcinoma, Squamous CellCyclin-Dependent Kinase Inhibitor p16Epstein-Barr Virus InfectionsFemaleHerpesvirus 4, HumanHumansMaleMiddle AgedOropharyngeal NeoplasmsPapillomaviridaePapillomavirus InfectionsPolyomavirusPolyomavirus InfectionsPrognosisTumor Virus InfectionsBiomarkers, TumorCDKN2A protein, humanCyclin-Dependent Kinase Inhibitor p16Epstein–Barr virusHuman papillomavirusOropharyngeal cancerPolyomavirusPrognosis

Identifiers

PMID32314041
PMCPMC7347695
OpenAlexW3016423154

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.