ArticleNature nanotechnology2020
Three-dimensional localization microscopy in live flowing cells.
Article in Nature nanotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Patch deconvolution for Fourier light-field microscopy.Biophysical journal · 2026Article
- LFC-Lab on a chip · 2026Article
- Super-Resolving Particle Diffusion Heterogeneity in Porous Hydrogels via High-Speed 3D Active-Feedback Single-Particle Tracking Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- High-throughput DNA repair monitoring in Saccharomyces cerevisiae suggests SSB- and DSB-induced chromatin reconfiguration.Scientific reports · 2025Article
- Super-resolving particle diffusion heterogeneity in porous hydrogels via high-speed 3D active-feedback single-particle tracking microscopy.bioRxiv : the preprint server for biology · 2025Article
- Simultaneous particle tracking, phase retrieval and point spread function reconstruction.bioRxiv : the preprint server for biology · 2025Article
- Femtosecond laser microfabrication of a fully-integrated optofluidic device for 3D imaging flow cytometry.Scientific reports · 2025Article
- Depth-enhanced high-throughput microscopy by compact PSF engineering.Nature communications · 2024Article
- Light-field flow cytometry for high-resolution, volumetric and multiparametric 3D single-cell analysis.Nature communications · 2024Article
- Imaging the scattered light of a nanoparticle through a cylindrical capillary.Nanophotonics (Berlin, Germany) · 2024Article
- Fast and parallel nanoscale three-dimensional tracking of heterogeneous mammalian chromatin dynamics.Molecular biology of the cell · 2022Article
- High-Throughput Imaging of CRISPR- and Recombinant Adeno-Associated Virus-Induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells.The CRISPR journal · 2022Article
- What is the future of electrical impedance spectroscopy in flow cytometry?Biomicrofluidics · 2021Article
- DNA-Based Microparticle Tension Sensors (μTS) for Measuring Cell Mechanics in Non-planar Geometries and for High-Throughput Quantification.Angewandte Chemie (International ed. in English) · 2021Article
- Self-contained and modular structured illumination microscope.Biomedical optics express · 2021Article
- 3D printable diffractive optical elements by liquid immersion.Nature communications · 2021Article
- Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine.Chemical Society reviews · 2021Review
- Choice of fluorophore affects dynamic DNA nanostructures.Nucleic acids research · 2021Article
- The frontier of live tissue imaging across space and time.Cell stem cell · 2021Review
- Axial localization and tracking of self-interference nanoparticles by lateral point spread functions.Nature communications · 2021Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capturing the dynamics of live cell populations with nanoscale resolution poses a significant challenge, primarily owing to the speed-resolution trade-off of existing microscopy techniques. Flow cytometry would offer sufficient throughput, but lacks subsample detail. Here we show that imaging flow cytometry, in which the point detectors of flow cytometry are replaced with a camera to record 2D images, is compatible with 3D localization microscopy through point-spread-function engineering, which encodes the depth of the emitter into the emission pattern captured by the camera. The extraction of 3D positions from sub-cellular objects of interest is achieved by calibrating the depth-dependent response of the imaging system using fluorescent beads mixed with the sample buffer. This approach enables 4D imaging of up to tens of thousands of objects per minute and can be applied to characterize chromatin dynamics and the uptake and spatial distribution of nanoparticles in live cancer cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.