ArticleNature communications2020
Nitrogen limitation reveals large reserves in metabolic and translational capacities of yeast.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 94 citations in OpenAlex.
- Metabolic engineering ofApplied and environmental microbiology · 2026Review
- Metabolic robustness is an emergent property of hierarchical network organization in yeast.mSystems · 2026Article
- Energy-translation coupling limits anaerobic yeast growth.Nature communications · 2026Article
- EnzymeTuning improves enzyme-constrained metabolic modeling and proteome abundance prediction through deep learning.Nature communications · 2026Article
- Accurate prediction of flux distributions compatible with metabolite concentration effects in genome-scale metabolic networks.PLoS computational biology · 2026Article
- Improved flux profiling in genome-scale modeling of human cell metabolism.Cell reports methods · 2026Article
- Glycolytic ATP production enables rapid mammalian cell growth.bioRxiv : the preprint server for biology · 2025Article
- Minimization of proteome reallocation explains metabolic transition in hierarchical utilization of carbon sources.mSystems · 2025Article
- Coarse-Grained Modeling Elucidates Differential Metabolism ofACS synthetic biology · 2025Article
- Thermal hydrolysis of poultry byproducts for the production of microbial media.Scientific reports · 2025Article
- Adaptive Laboratory Evolution and Carbon/Nitrogen Imbalance Promote High-Yield Ammonia Release inMicroorganisms · 2025Article
- Modeling for understanding and engineering metabolism.QRB discovery · 2025Article
- Yeast9: a consensus genome-scale metabolic model for S. cerevisiae curated by the community.Molecular systems biology · 2024Article
- Machine learning of metabolite-protein interactions from model-derived metabolic phenotypes.NAR genomics and bioinformatics · 2024Article
- An economic demand-based framework for prioritization strategies in response to transient amino acid limitations.Nature communications · 2024Article
- CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome.Microbial cell factories · 2024Article
- Identification of Protein-Protein Interaction Associated Functions Based on Gene Ontology.The protein journal · 2024Article
- Shaping of microbial phenotypes by trade-offs.Nature communications · 2024Review
- Increased CONature communications · 2024Article
- Imbalanced metabolism induced NHInternational microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwise reducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and transcript content. Combining multi-omics analysis with metabolic modeling, we find that seven metabolic superpathways maintain >50% metabolic capacity in reserve, with glucose metabolism maintaining >80% reserve capacity. Cells maintain >50% reserve in translational capacity for 2490 out of 3361 expressed genes (74%), with a disproportionately large reserve dedicated to translating metabolic proteins. Finally, ribosome reserves contain up to 30% sub-stoichiometric ribosomal proteins, with activation of reserve translational capacity associated with selective upregulation of 17 ribosomal proteins. Together, our dataset provides a quantitative link between yeast physiology and cellular economics, which could be leveraged in future cell engineering through targeted proteome streamlining.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.