ArticleParasites & vectors2020
Fast multiplex real-time PCR assay for simultaneous detection of dog and human blood and Leishmania parasites in sand flies.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- SERS-based spectral analysis of ITS1 PCR products for differentiation of three old world Leishmania species.Lasers in medical science · 2026Article
- Integrating molecular tools into leishmaniosis surveillance: evaluation of a commercial qPCR kit in sand fly vectors.Parasites & vectors · 2026Article
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- A RNase H2-Linked TaqMan-MGB Quantitative Real-Time PCR Assay for Differential Detection ofTransboundary and emerging diseases · 2026Article
- Rapid 65-min SYBR-Green PCR Assay for Carbapenem ResistantMicroorganisms · 2025Article
- A Fast TaqManGenes · 2025Article
- Advances and Challenges in the Diagnosis of Leishmaniasis.Molecular diagnosis & therapy · 2025Review
- A one-step multiplex qPCR assay for simultaneous identification and quantification of Leishmania martiniquensis and Leishmania orientalis/Leishmania chancei and detection and quantification of trypanosomatids in clinical samples.Parasite (Paris, France) · 2025Article
- Article
- Invasive Haemaphysalis longicornis (Acari: Ixodidae) investigation in South Carolina: new records of establishment, pathogen prevalence, and blood meal analyses.Journal of medical entomology · 2023Article
- Effective immuno-therapeutic treatment of Canine Leishmaniasis.PLoS neglected tropical diseases · 2023Article
- Concurrent molecular characterization of sand flies and Leishmania parasites by amplicon-based next-generation sequencing.Parasites & vectors · 2022Article
- No molecular evidence of SARS-CoV-2 infection in companion animals from Veracruz, Mexico.Transboundary and emerging diseases · 2022Article
- Leishmania V. braziliensis infection in asymptomatic domestic animals within an endemic region in the Northeast of Brazil.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Review
- Evaluation of different storage times and preservation methods on phlebotomine sand fly DNA concentration and purity.Parasites & vectors · 2020Article
- Molecular diagnosis of infectious parasites in the post-COVID-19 era.Tropical parasitologyArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundThe blood-feeding behaviour of female sand flies may increase their likelihood of acquiring and transmitting Leishmania parasites. Studies on the host usage by these insects may thus improve our understanding of the Leishmania transmission risk in leishmaniasis-endemic areas. Here, we developed a fast multiplex real-time PCR assay for simultaneous detection of dog, human and Leishmania DNA in sand flies.
methodsPrimers and TaqMan probes targeting the mitochondrial cytochrome c oxidase subunit 1 and cytochrome b genes of dog and human, respectively, were combined in a multiplex assay, which also includes primers and a TaqMan probe targeting the Leishmania minicircle kinetoplast DNA.
resultsThe multiplex assay was 100% specific, with analytical sensitivities of 10
conclusionsThis multiplex real-time PCR assay represents a novel fast assay for detecting dog, human and Leishmania DNA in female sand flies and therefore a tool for assessing the risk of Leishmania transmission to these hosts in areas of active transmission.
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