ArticleAnnals of laboratory medicine2020
Specific IgG and IgA Antibody Reactivities in Sera of Children by Enzyme-Linked Immunoassay and Comparison With Giardia duodenalis Diagnosis in Feces.
Article in Annals of laboratory medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- β-Giardin as an Immunomagnetic Enrichment Target for Multi-Host Detection ofPathogens (Basel, Switzerland) · 2025Article
- Coproantigen detection and molecular identification of Cryptosporidium species among newborn and adult farm animals.AMB Express · 2025Article
- Correlation between Melatonin and Colostral Regulatory T Cells inBiomolecules · 2024Article
- Detection of IgG Anti-Giardia duodenalis Antibodies in Sera by Indirect Immunofluorescence and Western Blotting Assays.Acta parasitologica · 2024Article
- Anti-Cryptosporidium oocysts polyclonal antibodies for cryptosporidiosis diagnosis and protection.AMB Express · 2023Article
- Role of anti-Giardia recombinant cyst wall protein IgG polyclonal antibodies in diagnosis and protection.AMB Express · 2022Article
- Utility of an Automatic Vision-Based Examination System (AVE-562) for the Detection ofAnnals of laboratory medicine · 2021Article
- Foodborne Parasites Dominate Current Parasitic Infections in Hunan Province, China.Frontiers in cellular and infection microbiology · 2021Article
- Antigen-Based Diagnosis of Human Giardiasis: A Systematic Review and Meta-Analysis.Iranian journal of parasitologyReview
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGiardia duodenalis is conventionally diagnosed in fecal samples using parasitological methods. However, sensitivity is poor when only a single sample is analyzed, due to intermittent excretion of cysts in feces. Alternatively, the serum antibodies to G. duodenalis can be used for parasite diagnosis and epidemiological studies to determine previous exposure. We compared the rate of G. duodenalis infection between serum anti-Giardia IgG and IgA antibodies and fecal examination in Brazilian children.
methodsFecal and serum samples were tested from 287 children at a clinical laboratory and from 187 children at daycare centers. Fecal samples were processed using conventional parasitological methods and coproantigen detection for Giardia diagnosis. Serum samples were tested using an in-house ELISA for detection of anti-Giardia IgG and IgA.
resultsG. duodenalis was found in 8.2% (N=39) of the 474 children analyzed. The sensitivity and specificity of ELISA were 80.0% and 90.0% for IgG and 80.0% and 83.3% for IgA, respectively. The total positivity rate of anti-Giardia IgG and IgA in the sera was 13.9% (N=66) and 23.6% (N=112). The agreement between the positivity of specific antibodies and the detection of G. duodenalis in feces was moderate for ELISA-IgG, kappa index (95% CI)=0.543 (0.422-0.664), and mild for ELISA-IgA, kappa index (95% CI)=0.283 (0.162-0.404). Among the children infected with other enteroparasites, 11.6% (N=10) and 24.4% (N=21) showed reactivity to anti-Giardia IgG and to IgA, respectively. This cross-reactivity was more frequent in samples from children infected with Endolimax nana and Entamoeba coli.
conclusionsThe higher frequency of specific antibody reactivity compared with G. duodenalis diagnosis in feces could reflect continuous exposure of children to G. duodenalis infection, resulting in long-lasting immunological memory and/or cross-reactivity with other intestinal amoebas.
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