ArticleThe EMBO journal2020
Polarized sorting of Patched enables cytoneme-mediated Hedgehog reception in the Drosophila wing disc.
Article in The EMBO journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- Emerging Roles of Cytoneme-Mediated Signaling in Cancer.International journal of molecular sciences · 2026Review
- Extracellular Vesicles in Arthropods: Biogenesis, Functions, Isolation Methods and Applications.Journal of extracellular vesicles · 2025Review
- Hedgehog pathway, cell cycle, and primary cilium.Cell death discovery · 2025Review
- Making cells inter-connected for signaling communication: a developmental view of cytonemes.Cell communication and signaling : CCS · 2025Review
- The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour.Nature communications · 2025Article
- Morphogens and Cell-Derived Structures (Exosomes and Cytonemes) as Components of the Communication Between Cells.International journal of molecular sciences · 2025Review
- Hijacking host cell vesicular transport: New insights into the nutrient acquisition mechanism ofVirulence · 2024Review
- Oxidative stress-induced changes in the transcriptomic profile of extracellular vesicles.Journal of extracellular biology · 2024Article
- Review
- Myosin XV is a negative regulator of signaling filopodia during long-range lateral inhibition.Developmental biology · 2024Article
- VAMP5 and distinct sets of cognate Q-SNAREs mediate exosome release.Cell structure and function · 2023Article
- Cancer-associated fibroblasts influence Wnt/PCP signaling in gastric cancer cells by cytoneme-based dissemination of ROR2.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Myosin XV is a negative regulator of signaling filopodia during long-range lateral inhibition.bioRxiv : the preprint server for biology · 2023Article
- The role of glycosaminoglycan modification in Hedgehog regulated tissue morphogenesis.Biochemical Society transactions · 2023Review
- Understanding the interplay of membrane trafficking, cell surface mechanics, and stem cell differentiation.Seminars in cell & developmental biology · 2023Review
- Predictive model for cytoneme guidance in Hedgehog signaling based on Ihog- Glypicans interaction.Nature communications · 2022Article
- Regulatory mechanisms of cytoneme-based morphogen transport.Cellular and molecular life sciences : CMLS · 2022Review
- Cholesterol and Hedgehog Signaling: Mutual Regulation and Beyond.Frontiers in cell and developmental biology · 2022Review
- The power of imaging to understand extracellular vesicle biology in vivo.Nature methods · 2021Review
- Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Hedgehog (Hh) signal molecules play a fundamental role in development, adult stem cell maintenance and cancer. Hh can signal at a distance, and we have proposed that its graded distribution across Drosophila epithelia is mediated by filopodia-like structures called cytonemes. Hh reception by Patched (Ptc) happens at discrete sites along presenting and receiving cytonemes, reminiscent of synaptic processes. Here, we show that a vesicle fusion mechanism mediated by SNARE proteins is required for Ptc placement at contact sites. Transport of Ptc to these sites requires multivesicular bodies (MVBs) formation via ESCRT machinery, in a manner different to that regulating Ptc/Hh lysosomal degradation after reception. These MVBs include extracellular vesicle (EV) markers and, accordingly, Ptc is detected in the purified exosomal fraction from cultured cells. Blockage of Ptc trafficking and fusion to basolateral membranes result in low levels of Ptc presentation for reception, causing an extended and flattened Hh gradient.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.