Evidence map›Paper›PMID 32309932›Full record

ArticleInorganic chemistry2020

Artificial Iron Proteins: Modeling the Active Sites in Non-Heme Dioxygenases.

Kelsey R Miller, Jonathan D Paretsky, Alec H Follmer, Tillmann Heinisch, Kaustuv Mittra, Sheraz Gul, In-Sik Kim, Franklin D Fuller, Alexander Batyuk, Kyle D Sutherlin and 8 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inorganic chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
  6. Review
  7. Artificial Metalloproteins with Dinuclear Iron-Hydroxido Centers.Journal of the American Chemical Society · 2021
    Article
  8. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources.Methods in molecular biology (Clifton, N.J.) · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 4 institutions in 2 countries.

Kelsey R MillerDepartment of Chemistry, 1102 Natural Science II, University of California, Irvine, California 92697, United States.
Jonathan D ParetskyDepartment of Chemistry, 1102 Natural Science II, University of California, Irvine, California 92697, United States.
Alec H FollmerDepartment of Chemistry, 1102 Natural Science II, University of California, Irvine, California 92697, United States.ORCID http://orcid.org/0000-0002-6244-6804
Tillmann HeinischDepartment of Chemistry, University of Basel, P.O. Box 3350, Mattenstrasse 24a, BPR 1096, CH-4002 Basel, Switzerland.
Kaustuv MittraDepartment of Molecular Biosciences and Biochemistry, University of California, Irvine, California 92697, United States.ORCID http://orcid.org/0000-0003-2325-1972
Sheraz GulMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
In-Sik KimMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Franklin D FullerLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, United States.
Alexander BatyukLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, United States.
Kyle D SutherlinMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Aaron S BrewsterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Asmit BhowmickMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Nicholas K SauterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Jan KernMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.ORCID http://orcid.org/0000-0002-7272-1603
Junko YanoMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.ORCID http://orcid.org/0000-0001-6308-9071
Michael T GreenDepartment of Chemistry, 1102 Natural Science II, University of California, Irvine, California 92697, United States.ORCID http://orcid.org/0000-0001-8658-8420
Thomas R WardDepartment of Chemistry, University of Basel, P.O. Box 3350, Mattenstrasse 24a, BPR 1096, CH-4002 Basel, Switzerland.ORCID http://orcid.org/0000-0001-8602-5468
A S BorovikDepartment of Chemistry, 1102 Natural Science II, University of California, Irvine, California 92697, United States.ORCID http://orcid.org/0000-0001-5049-9952
Lawrence Berkeley National Laboratory · USUniversity of California, Irvine · USSLAC National Accelerator Laboratory · USUniversity of Basel · CH

Funding

DIALS: New Computational Methods to Enable Challenging Crystallographic ExperimentsR01GM117126 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2016 to 2023
$6.1M
Biological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Supplemental EquipmentR01GM110501 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Junko Yano · 2014 to 2026
$4.8M
High throughput sample delivery method for time resolved studies of enzyme reactions with X-ray and complementary techniquesR01GM126289 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI KERN, JAN F · 2017 to 2025
$4.6M
Thermodynamic, Electronic, Structural, and Kinetic Characterizations of Cytochrome P450 Compounds I & IIR01GM101390 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI MICHAEL T. GREEN · 2012 to 2026
$3.0M
Confining Metal Complexes within Protein Hosts: Models for Metalloprotein Active SitesR01GM120349 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI BOROVIK, ANDREW S. · 2017 to 2024
$2.4M
High-end X-ray Detector System for Femtosecond CrystallographyS10OD023453 · OD · STANFORD UNIVERSITY · PI BOUTET, SEBASTIEN · 2017 to 2017
$1.7M
NIGMS NIH HHS R01 GM101390NIGMS NIH HHS R01 GM110501NIGMS NIH HHS R01 GM117126NIGMS NIH HHS R01 GM120349NIGMS NIH HHS R01 GM126289NIH HHS S10 OD023453
6 · The paper itself

Abstract

An important class of non-heme dioxygenases contains a conserved Fe binding site that consists of a 2-His-1-carboxylate facial triad. Results from structural biology show that, in the resting state, these proteins are six-coordinate with aqua ligands occupying the remaining three coordination sites. We have utilized biotin-streptavidin (Sav) technology to design new artificial Fe proteins (ArMs) that have many of the same structural features found within active sites of these non-heme dioxygenases. An Sav variant was isolated that contains the S

Indexed as

Binding SitesDioxygenasesFerric CompoundsLigandsMolecular ConformationNonheme Iron ProteinsDioxygenasesFerric CompoundsLigandsNonheme Iron Proteins

Identifiers

PMID32309932
PMCPMC7219546
OpenAlexW3016753178

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.