ArticleACS omega2020
Comparison of Colorimetric Analyses to Determine Cortisol in Human Sweat.
Article in ACS omega, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The trial behind it
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Who cites it
24 citing papers in PubMed, 81 citations in OpenAlex.
- Advances and Challenges in Wearable Sensors for Health Monitoring.ACS applied materials & interfaces · 2026Review
- A quantitative, multimodal wearable bioelectronic device for comprehensive stress assessment and sub-classification.Nature communications · 2026Article
- Modulating Role of Resveratrol in Metabolic and Inflammatory Dysregulation Caused by Surgical and Psychoemotional Stress in Rats.Pathophysiology : the official journal of the International Society for Pathophysiology · 2025Article
- Article
- A Colorimetric Approach for Detecting a Selected Fecal Cortisol Metabolite as a Stress Biomarker in Atlantic Salmon.ACS omega · 2025Article
- Recent Advances on Rapid Detection Methods of Steroid Hormones in Animal Origin Foods.Biosensors · 2025Review
- Emerging Dual-Gate FET Sensor Paradigm for Ultra-Low Concentration Cortisol Detection in Complex Bioenvironments.Biosensors · 2025Article
- Electrochemical Biosensors by Means of Molecularly Imprinted Polymers (MIPs) Cortisol Recognition.Polymers · 2025Article
- Rapid optical determination of salivary cortisol responses in individuals undergoing physiological and psychological stress.Scientific reports · 2024Article
- Enzyme-free immunoassay for rapid, sensitive, and selective detection of C-reactive protein.Analytical and bioanalytical chemistry · 2024Article
- Human Activity Recording Based on Skin-Strain-Actuated Microfluidic Pumping in Asymmetrically Designed Micro-Channels.Sensors (Basel, Switzerland) · 2024Article
- MicroSweat: A Wearable Microfluidic Patch for Noninvasive and Reliable Sweat Collection Enables Human Stress Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A "Two-Part" Resonance Circuit Based Detachable Sweat Patch for Noninvasive Biochemical and Biophysical Sensing.Advanced functional materials · 2023Article
- Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat.Biosensors · 2023Article
- Article
- Nano gold-doped molecularly imprinted electrochemical sensor for rapid and ultrasensitive cortisol detection.Biosensors & bioelectronics · 2022Article
- Enzymatic Methods for Salivary Biomarkers Detection: Overview and Current Challenges.Molecules (Basel, Switzerland) · 2021Review
- UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction.Molecules (Basel, Switzerland) · 2021Article
- Immunosensor for Assessing the Welfare of Trainee Guide Dogs.Biosensors · 2021Article
- State of Sweat: Emerging Wearable Systems for Real-Time, Noninvasive Sweat Sensing and Analytics.ACS sensors · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Colorimetric analysis, which relies on a chemical reaction to facilitate a change in visible color, is a great strategy for detecting cortisol, which is necessary to diagnose and manage the wide variety of diseases related to the hormone, because it is simple in design, inexpensive, and reliable as a standard cortisol analysis technique. In this study, four different colorimetric cortisol analyses that use various chromogens, which include sulfuric acid, Porter-Silber reagent, Prussian blue, and blue tetrazolium, are studied. Modifications to the classic Porter-Silber method are made by increasing the carbon content of the alcohol and adding gold nanoparticles, which result in a twofold increase in reaction rate and a slight decrease in the limit of detection (LoD). After a comparison of the reaction rate, LoD, dynamic range, characteristic peaks, and color stability of all methods, blue tetrazolium demonstrates a low LoD (97 ng/mL), broad dynamic range (0.05-2 μg/mL), and quick reaction rate (color development as fast as 10 min), which are well within the requirements for human biofluids. Cortisol in artificial saliva and sweat and in human sweat was determined while confirming that no excipients or other biomarkers interfered with the reactions. Twenty-one human sweat samples were tested using blue tetrazolium and revealed a significant difference between male and female apocrine cortisol concentrations and showed a highly significant difference between apocrine and eccrine cortisol concentrations. Colorimetric methods of cortisol can compete with existing electrochemical sensors because of their similar accuracy and detection range in certain wearable biosensor applications. The simplicity of colorimetric methods advances potential applications in skin-interfaced bio-electronics and point-of-care devices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.