ArticleACS omega2020
Direct Ultraviolet Laser-Induced Reduction of Disulfide Bonds in Insulin and Vasopressin.
Article in ACS omega, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Single-Cell Nucleus Extraction with Cellular Indexing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Light-driven biosensor for the rapid and selective detection of hypoxia-inducible factor-prolyl hydroxylase domain inhibitors in aqueous media and saliva.Mikrochimica acta · 2025Article
- Oxygen-Tolerant, Red Light-Driven Controlled Synthesis of Easily Degradable and High Molecular Weight α-Lipoic Acid-Vinyl Copolymers.Angewandte Chemie (International ed. in English) · 2025Article
- Grafted Disulfide-Linked Linear Polymers for Heterogeneous Thiyl Radical Photocatalysis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Size-Dependent Ultrafast UV Photochemistry of Aliphatic Disulfides in Solution.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Review
- Top-down mass spectrometry and assigning internal fragments for determining disulfide bond positions in proteins.The Analyst · 2022Article
- Electrocatalytic Depolymerization of Self-Immolative Poly(Dithiothreitol) Derivatives.Molecules (Basel, Switzerland) · 2022Article
- Redox chemistry of lens crystallins: A system of cysteines.Experimental eye research · 2021Review
- Effect of Addition of Tryptophan on Aggregation of Apo-α-Lactalbumin Induced by UV-Light.Foods (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ultraviolet (UV) light has been shown to induce reduction of disulfide bonds in proteins in solution. The photoreduction is proposed to be a result of electron donation from excited Tyr or Trp residues. In this work, a powerful UV femtosecond laser was used to generate photoreduced products, while the hypothesis of Tyr/Trp mediation was studied with spectroscopy and mass spectrometry. With limited irradiation times of 3 min or less at 280 nm, the laser-induced reduction in arginine vasopressin and human insulin led to significant yields of ∼3% stable reduced product. The photogenerated thiols required acidic pH for stabilization, while neutral pH primarily caused scrambling and trisulfide formation. Interestingly, there was no direct evidence that Tyr/Trp mediation was a required criterion for the photoreduction of disulfide bonds. Intermolecular electron transfer remained a possibility for insulin but was ruled out for vasopressin. We propose that an additional mechanism should be increasingly considered in UV light-induced reduction of disulfide bonds in solution, in which a single UV photon is directly absorbed by the disulfide bond.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.