Evidence map›Paper›PMID 32309616›Full record

ArticleDiscoveries (Craiova, Romania)2019

Nanoscale imaging of E. coli cells by expansion microscopy.

Sharey Cheng, Yongxin Zhao

Open access · diamondAbstract read
In one paragraph

Article in Discoveries (Craiova, Romania), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Sharey ChengDepartment of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA.
Yongxin ZhaoDepartment of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA.
Carnegie Mellon University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expansion microscopy (ExM) is an emerging super-resolution imaging technology. ExM works by infusing a biological specimen with a superabsorbent hydrogel, followed by mechanical homogenization and isotropical expansion of the specimen in water. The unique and cost-effective process of ExM enables super-resolution optical imaging of sample of interest without the need to invest and use of a sophisticated microscope instrument. Here, we demonstrate that a nearly 3-fold isotropic physical expansion of E.coli fixed cells can be achieved in PBS, and the cell morphology during binary fission is clearly resolved in the expanded state, using a diffraction-limited microscope.

Indexed as

bacteriaEscherichia coliExpansion Microscopysuper-resolution imaging.

Identifiers

PMID32309616
PMCPMC7086062
OpenAlexW2978243651

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.