ArticlePlastic and reconstructive surgery. Global open2020
A 3-step Mechanical Digestion Method to Harvest Adipose-derived Stromal Vascular Fraction.
Article in Plastic and reconstructive surgery. Global open, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 4 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 4 syntheses or guidelines pooled it, 35 citations in OpenAlex.
- Mechanical isolation of stromal vascular fraction from adipose tissue: methods and cellular outcomes: a systematic review and meta-analysis.Stem cell research & therapy · 2025Pooled it
- The efficiency of human fat products in wound healing: A systematic review and meta-analysis.International wound journal · 2024Pooled it
- Pooled it
- Analyzing the Clinical Potential of Stromal Vascular Fraction: A Comprehensive Literature Review.Medicina (Kaunas, Lithuania) · 2024Pooled it
- Mechanical isolation of stromal vascular fraction using a spiral-based device: cellular outcomes and point-of-care workflow considerations.Journal of biological engineering · 2026Article
- Uniform Treatment Protocol With Hybrid Stromal Vascular Fraction and Platelet-Rich Plasma on Micro-Fat Grafting With Facelifting: A Preliminary Retrospective 3D Vectra Observational Study.Aesthetic surgery journal. Open forum · 2026Article
- Adipose tissue as a living drug: Stromal vascular fraction and adipose tissue-derived stem cells in regenerative medicine.World journal of stem cells · 2025Review
- A New Era of Regenerative Medicine, With Longevity as the Cornerstone.HSS journal : the musculoskeletal journal of Hospital for Special Surgery · 2025Article
- Clinical Applications of Stromal Vascular Fraction and Stromal Vascular Matrix for Osteoarthritis: A Commentary.HSS journal : the musculoskeletal journal of Hospital for Special Surgery · 2025Article
- Topical Application of SVF/PRF in Thermal Injuries-A Retrospective Analysis.Journal of clinical medicine · 2025Article
- A modified technique for mechanical isolation of stromal vascular fraction yields increased final product volume and high viable nucleated cells count.Journal of experimental orthopaedics · 2025Article
- Innovative Mechanical Processing of Adipose Tissue: A Comparative Evaluation of Microfat and Concentrated Micronized Fat for Superficial Fat Grafting.Aesthetic plastic surgery · 2025Article
- Article
- Unilocular adipocyte and lipid tracer for immunofluorescent images.Scientific reports · 2025Article
- Review
- Advanced methods to mechanically isolate stromal vascular fraction: A concise review.Regenerative therapy · 2024Review
- AI-enhanced "Two-thirds Guidelines" for Lipolifting: Addressing Multiple Hallmarks of Facial Aging.Plastic and reconstructive surgery. Global open · 2024Article
- A Simple and Effective Mechanical Method for Adipose-Derived Stromal Vascular Fraction Isolation.Cureus · 2024Article
- A Multivariate Meta-Analysis for Optimizing Cell Counts When Using the Mechanical Processing of Lipoaspirate for Regenerative Applications.Pharmaceutics · 2023Article
- Mechanical Fractionation of Adipose Tissue-A Scoping Review of Procedures to Obtain Stromal Vascular Fraction.Bioengineering (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdipose stromal vascular fraction (SVF) isolation with enzymatic digestion is the gold standard, but is expensive, having practical and legal concerns. The alternative mechanical SVF isolation methods provide lower cell yields as they employ either centrifugation, emulsification, or digestion steps alone. We combined mechanical processing with buffer incubation and centrifugation steps into an isolation method called "mechanical digestion" and compared the cell yields with that of enzymatic digestion.
methodsA total of 40-mL lipoaspirate was harvested from 35 women undergoing liposuction and was submitted to conventional enzymatic digestion for SVF isolation or mechanical digestion using a closed unit harnessing 3 ports with blades, followed by buffer incubation and centrifugation. Culture of the SVFs and flow cytometry were performed.
resultsThe SVF cell yield obtained by enzymatic digestion was significantly higher 3.38 × 10
conclusionsMechanically digested SVF was comparable to enzymatically digested SVF in terms of stromal cell composition and viability. With mechanical digestion, we can isolate 30%-50% SVF cells of that isolated with enzymatic digestion. Further studies are warranted to determine the clinical outcomes.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.