ArticleBiotechnology for biofuels2020
Enzymatic conversion reactions of 5-hydroxymethylfurfural (HMF) to bio-based
Article in Biotechnology for biofuels, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 75 citations in OpenAlex.
- Biocatalytic Production of Oxidized Furan Derivatives from Lignocellulosic Sugar-Rich Streams Using a Novel Fungal Glyoxal Oxidase.Journal of agricultural and food chemistry · 2026Article
- Advance biocatalyst for the green conversion of 5-hydroxymethylfurfural to a biopolymer precursor 2,5-furandicarboxylic acid.Biotechnology for biofuels and bioproducts · 2025Article
- Article
- Selective and green conversion of 5-HMF to FDCARSC advances · 2025Article
- Manganese-catalyzed oxidation of furfuryl alcohols and furfurals to efficient synthesis of furoic acids.RSC advances · 2024Article
- Hematin and CuiScience · 2023Article
- Spectrophotometric Assay for the Detection of 2,5-Diformylfuran and Its Validation through Laccase-Mediated Oxidation of 5-Hydroxymethylfurfural.International journal of molecular sciences · 2023Article
- Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase.Biotechnology for biofuels and bioproducts · 2023Article
- Enzymatic reactions towards aldehydes: An overview.Flavour and fragrance journal · 2023Review
- 2,5-Furandicarboxaldehyde as a bio-based crosslinking agent replacing glutaraldehyde for covalent enzyme immobilization.RSC advances · 2022Article
- Current Advances in the Sustainable Conversion of 5-Hydroxymethylfurfural into 2,5-Furandicarboxylic Acid.ChemSusChem · 2022Review
- Catalytic Furfural/5-Hydroxymethyl Furfural Oxidation to Furoic Acid/Furan-2,5-dicarboxylic Acid with HJournal of the American Chemical Society · 2022Article
- Nanocomposite films as electrochemical sensors for detection of catalase activity.Frontiers in molecular biosciences · 2022Article
- Peptide conformational imprints enhanced the catalytic activity of papain for esterification.Frontiers in bioengineering and biotechnology · 2022Article
- Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering.Nature communications · 2021Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background2,5-Furandicarboxylic acid (FDCA) is one of the top biomass-derived value-added chemicals. It can be produced from fructose and other C6 sugars via formation of 5-hydroxymethilfurfural (HMF) intermediate. Most of the chemical methods for FDCA production require harsh conditions, thus as an environmentally friendly alternative, an enzymatic conversion process can be applied.
resultsCommercially available horseradish peroxidase (HRP) and lignin peroxidase (LPO), alcohol (AO) and galactose oxidase (GO), catalase (CAT) and laccase (LAC) were tested against HMF, 2,5-diformylfuran (DFF), 5-hydroxymethyl-2-furoic acid (HMFA) and 5-formyl-2-furoic acid (FFA). Enzyme concentrations were determined based on the number of available active sites and reactions performed at atmospheric oxygen pressure. AO, GO, HRP and LPO were active against HMF, where LPO and HRP produced 0.6 and 0.7% of HMFA, and GO and AO produced 25.5 and 5.1% DFF, respectively. Most of the enzymes had only mild (3.2% yield or less) or no activity against DFF, HMFA and FFA, with only AO having a slightly higher activity against FFA with an FDCA yield of 11.6%. An effect of substrate concentration was measured only for AO, where 20 mM HMF resulted in 19.5% DFF and 5 mM HMF in 39.9% DFF, with a
conclusionsOur study aimed at understanding the mechanism of conversion of bio-based HMF to FDCA by different selected enzymes. By understanding the reaction pathway, as well as substrate specificity and the effect of substrate concentration, we would be able to better optimize this process and obtain the best product yields in the future.
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